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In this work, we propose an efficient numerical method for computing the electrostatic interaction between two like-charged spherical particles which is governed by the nonlinear Poisson-Boltzmann equation. The nonlinear problem is solved by a monotone iterative method which leads to a sequence of linearized equations. A modified central finite difference(More)
Violation of energy conservation in Poisson-Boltzmann molecular dynamics, due to the limited accuracy and precision of numerical methods, is a major bottleneck preventing its wide adoption in biomolecular simulations. We explored the ideas of enforcing interface conditions by the immerse interface method and of removing charge singularity to improve the(More)
Spatial neighbouring relation is a very important concept in spatial information science. It has even been regarded as the base of spatial information system. Neighouring information is indispensable for many operations in spatial information system. Therefore, it is significant to describe and acquire such in an efficient way. Most of current concepts of(More)
Based on the investigation of the Huanghai Sea and the Bohai Sea in Spring( April to May) of 2014, the concentrations of urea and inorganic nitrogen were determined respectively by diacetyl monoxime-Semicarbazide Hydrochloride method and Spectrophotometric method. The distribution of urea and the component of dissolved nitrogen were analyzed. The(More)
The M and NP genes of H5N1 avian influenza virus (A/chicken/Hubei/489/2004) were amplified by RT-PCR from viral RNA, and cloned into pMD18-T vector respectively. The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote expression vector; the constructed plasmid(More)
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