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Nucleoplasmin regulates chromatin condensation during apoptosis.
- Zhi Gang Lu, Chuanmao Zhang, Z. Zhai
- BiologyProceedings of the National Academy of Sciences…
- 22 February 2005
It is shown that apoptotic chromatin condensation is regulated by nucleoplasmin, a protein that decondenses sperm chromatin during male pronuclear assembly, and that chromatincondensation and DNA fragmentation are independent processes.
Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-α/β
It is reported that chromatin-bound NLS (nuclear localization sequence) proteins provide docking sites for the NE precursor membrane vesicles and nucleoporins via importin-α and -β during NE assembly in Xenopus egg extracts.
Role of ran GTPase in cell cycle regulation
The mechanisms by which Ran and its interacting proteins regulate NE assembly, DNA replication and spindle assembly are discussed.
LDFF, the large molecular weight DNA fragmentation factor, is responsible for the large molecular weight DNA degradation during apoptosis in Xenopus egg extracts
Results indicate that LDFF in Xenopus egg extracts might be a new DNase (or DNases) responsible for the large DNA fragmentation, which is not the Xenopus CAD homolog XCAD.
Coexpression of DNA Fragmentation Factor Subunits in E.coli by Two Incompatible Plasmids.
- Wei Yang, Lan-Xin Zhang, Zhi Gang Lu, Z. Zhai
- Biology, ChemistrySheng wu hua xue yu sheng wu wu li xue bao Acta…
- 1 December 2001
A novel method of coexpressing different proteins using two incompatible plasmids was developed and the cotransformant was cultured in liquid medium containing ampicillin and kanamycin for 14 h, and more than 75% of the cells were found to be resistant to the two antibiotics, that is, they carried both pET21a-DFF45 and pET28a-dFF40.
Recombinant Human DFF45 Inhibits Apoptosis-specific Endonuclease in a Cell-free System of Xenopus Egg Extracts.
- Wei Yang, Zhi Gang Lu, Lan-Xin Zhang, Z. Zhai
- BiologySheng wu hua xue yu sheng wu wu li xue bao Acta…
- 1 December 2001
Purified recombinant human DFF45, added into the apoptotic cell-free system of Xenopus egg extracts, could effectively inhibit both the digestion of lambdaDNA and the degradation of chromosomal DNA into nucleosomal fragments in the nuclei of chicken red blood cells.
Specific degradation of keratin in Xenopus laevis egg extracts undergoing apoptosis
Cytochrome c activates apoptosis specific protease XCPP32 when being added to Xenopus laevis egg extracts, and induces apoptosis in this cell-free system. During apoptosis, cyto-skeleton proteins in…
Identification of a DNase activated inXenopus egg extracts undergoing apoptosis
A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150 and experiments showed that 27 ku nuclease which was different from other Ca2+ /Mg2+ -dependent nucleases was a possible candidate involved in apoptosis.
Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract
The results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku, and indicated that the pathway in apoptosis was conserved in evolution.
Observation of Chromosome Structure in Allium Cepa Cells by Means of Electron Microscopy
The results present new evidence to support the radial loop and coils coexist mode of chromosome and may more possibly accord with the real chromosome structure.