Zhenghua Song

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A sensitive flow injection-chemiluminescence (FI-CL) method for the determination of isocarbophos (ICP) residue on tangerines and oranges was proposed. It was found that the CL intensity from luminol-albumin CL reaction could be obviously quenched in the presence of ICP and the decrease in CL intensity was proportional to the logarithm of ICP concentrations(More)
A rapid and sensitive chemiluminescence (CL) determination of puerarin with the flow injection technique was presented. It was found that puerarin could enhance the CL generated from luminol-KIO(4) system significantly. The increment of CL intensity was linear over the concentration of puerarin ranging from 0.3 to 100.0 ng mL(-1) (r(2)=0.9963), offering a(More)
A sensitive flow injection chemiluminescence method, based on the inhibitory effect of chlorogenic acid on the reaction between luminol and dissolved oxygen, was presented for the determination of chlorogenic acid. It was found that the decrease of chemiluminescence intensity was linear with the logarithm of chlorogenic acid concentration over the range(More)
The interaction of lysozyme with bromophenol blue (BPB) in acetate buffer (pH 6.0) was studied by fluorescence quenching method for the first time. It was found that BPB could conspicuously quench the fluorescence of lysozyme by the static quenching process, possibly due to the binding on the active site near Trp62. The binding parameters including the(More)
  • Z Song, X Zhou
  • 2001
A novel chemiluminescence (CL) sensor for folic acid combined flow-injection (FI) technology was presented in this paper. The analytical reagents involved in the CL reaction, including luminol and hexacyanoferrate(III), were both immobilized on an anion-exchange column in FI system. The CL signal produced by the reaction between luminol and(More)
The luminol-bovine serum albumin chemiluminescence system was proposed for the first time. It was found that the hydrophilic luminol bound to the hydrophilic domain at Trp(134) of BSA with accelerating the electrons transferring rate of excited 3-aminophthalate, which led to the enhancement CL intensity of luminol at 425 nm. The increment of(More)
It was found that macromolecular complexes were formed between lysozyme and bromophenol blue (BPB) with the electrostatic attraction in acetate medium (pH 6.5). The binding constant and the number of binding site for lysozyme-BPB complex were obtained, and the thermodynamic parameters were given. In addition, a remarkable enhancement of resonance light(More)
It was first found that the intrinsic fluorescence of lysozyme at 340 nm can be quenched by cephalosporin analogues through the static quenching and non-radiative energy transferring procedure. In the acetate buffer solution with pH 7.0 and 298 K, the quenching fluorescence intensity was in a good linearity over the concentration of drugs in the range of(More)
A new flow injection method for the determination of folic acid is described. A fast oxidation reaction occurred when folic acid was mixed with potassium ferricyanide generating ferrocyanide which then inhibited the chemiluminescent reaction of ferricyanide and luminol in alkaline medium. The decrease of chemiluminescence intensity was correlated with the(More)
An interesting inhibitory effect of rutin on the chemiluminescence (CL) reaction between luminol and periodate was reported, and this effect was used for the determination of rutin in medicine and human urine. The CL reagents, luminol and periodate, were both immobilized on an anion-exchange column. The CL signal produced by the reaction between luminol and(More)