Zhengbo Liu

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Flavonoids are important polyphenolic secondary metabolites in plant. Citrus reticulata 'Chachi' fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. A high performance liquid chromatography-photodiode array detection system was used to analyze five flavonoids, namely,(More)
Water-soluble salvianolic acid A (Sal A) and salvianolic acid B (Sal B) were successfully isolated and purified from the crude extract of Salvia miltiorrhiza by high-speed counter-current chromatography (HSCCC). The solvent system was n-hexane-ethyl acetate-methanol-water (3:6:6:10, v/v/v/v). 4.27 mg of Sal A and 32.09 mg of Sal B were obtained from 260 mg(More)
Dihydrochalcones are the main active components of Lithocarpus polystachyus Rehd. (Sweet Tea), they are directly related to the sweet tonic beverage and traditional herb. In this work, two runs of preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane/ethyl acetate/ethanol/water (1:4:3:4, v/v) were(More)
INTRODUCTION Cynanchum bungei Decne (Baishouwu in China), is a famous traditional Chinese medicine that has been widely used as a tonic medicine or health food for centuries. Bungeiside-A, bungeiside-B and baishouwubenzophenone, as the major bioactive constituents in C. bungei, are challenging to separate and purify since bungeiside-A and -B are present in(More)
In this study, an aqueous ionic liquid based ultrasonic assisted extraction (ILUAE) method for the extraction of the four acetophenones, namely 4-hydroxyacetophenone (1), 2,5-dihydroxyacetophenone (2), baishouwubenzophenone (3) and 2,4-dihydroxyacetophenone (4) from the Chinese medicinal plant Cynanchum bungei was developed. Three kinds of aqueous(More)
Supercritical fluid extraction (SFE) was used to extract saikosaponins a, c and d from the root of Bupleurum falcatum. An orthogonal array design L(9)(3)(4) was employed as a chemometric method for the optimization of the SFE conditions. The effects of four factors including pressure (30-40 MPa), temperature (40-50 degrees C), ethanol concentration(More)
Accelerated solvent extraction (ASE) was applied to the extraction of saikosaponin a, saikosaponin c and saikosaponin d from the roots of Bupleurum falcatum. Main extraction parameters such as the extraction solvents, extraction temperature and static extraction time were investigated and optimized. The optimized procedure employed 70% methanol as(More)
Three flavones were prepared, isolated and purified from Pericarpium Citri Reticulatae by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (2:4:3:3, v/v/v/v) was used. Within 6 hours, 10.1 mg of heseridin, 49.8 mg of 5,6,7,8,4'-pentamethoxyflavone and 50.6 mg of(More)
OBJECTIVE To investigate clinical features and antifungal therapeutic effect of chronic severe hepatitis (CSH) patients with invasive fungal infection (IFI), and to improve the diagnosis and treatment. METHODS Clinical manifestation, blood routine, imageology and mycetology characteristic, antifungal treatment perscription and therapeutic effect of 79 CSH(More)
A high performance liquid chromatographic (HPLC) method was developed for the determination of four acetophenones, namely 4-hydroxyacetophenone (I), 2,5-dihydroxyacetophenone (II), baishouwubenzophenone (III) and 2,4-dihydroxyacetophenone (IV), in Radix Cynanchi bungei. The HPLC with photodiode array (PDA) detection was carried out on a Symmetry-C18 (4.6 mm(More)