Zheng-yan Yu

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Glucocorticoids or the glucocorticoid analog dexamethasone (DEX) enhances the differentiation of preadipocytes in the presence of insulin and influences preadipocyte proliferation. The purpose of the present study was to determine if DEX can induce the recruitment of preadipocytes. Using monoclonal antibodies for complement-mediated cytotoxicity,(More)
OBJECTIVE To determine if subpopulations of cells in stromal vascular (S-V) cultures could be segregated and separated based on affinity for laminin substratum. RESEARCH METHODS AND PROCEDURES S-V cells were seeded and allowed to attach for various times; 4 hours was found to be optimal for cell attachment. Cultures were rinsed after 4 hours of seeding,(More)
To observe the effect of hyperoxia on the growth of type II alveolar epithelial cells (AEC II). The lungs of 19-day gestation fetal rats were primary cultured and the AEC II were purified by differential adhesion method. The cells were divided into control (normoxia) group and hyperoxia group. The cell growth, cell viability, cell apoptosis, and cell cycle(More)
The expression of three CCAAT/enhancer-binding proteins (C/EBPs) was examined with immunocytochemistry and Western blot analysis during preadipocyte differentiation in porcine stromal vascular (S-V) cell cultures. Regardless of treatment and time in culture, immunoreactivity for all three C/EBP isoforms was restricted to cell nuclei. At day 1, 50 +/- 6% of(More)
At 90 days of gestation hypophysectomized (hypox) porcine fetuses were treated with hydrocortisone (HC) or thyroxine (T4) and S-V cultures prepared from intact, treated and untreated hypox fetuses at 105 days of gestation. After 24 h, cultures were stained for the AD-3 antigen and the transcription factors, C/EBP alpha and delta. The proportion of(More)
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