Zheng-ling Shang

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OBJECTIVE To test the effect of Rv0901 gene of Mycobacterium tuberculosis on the activity of mice macrophages. METHODS Peritoneal macrophages of mice were isolated and transfected with pcDNA3. 1 or pcDNA3. 1-Rv0901 plasmid DNA, along with the GFP DNA. The effectiveness of the transfection was detected by RT-PCR. The expression of the GFP and the apoptosis(More)
OBJECTIVE The mechanism by which M.tuberculosis persists and survives in host macrophage is not fully understood, however, the M. tuberculosis chromosome-encoded TA loci perform functions possibly of signaling to these processes. To explore the biological functions of M. tuberculosis chromosome-encoded TA loci, the Rv1494 and Rv1495 genes of M.tuberculosis(More)
OBJECTIVE To investigate the protein-protein interaction between hypothetical protein Rv1246c and Rv1247c of Mycobacterium tuberculosis. METHODS By PCR technique, the complete open-reading frame sequences of Rv1246c and Rv1247c gene were amplified from the M. tuberculosis H37Rv genomic DNA as template. The PCR-amplified cDNAs of Rv1247c and Rv1246c gene(More)
OBJECTIVE To investigate whether the RelE toxin protein of mycobacterium tuberculosis has a growth inhibition effect on lung cancer A-549 cell. METHODS The complete open-reading frame sequences of RelE, RelB and RelBE genes were amplified by PCR with using M. tuberculosis H37Rv genomic DNA as the template. The RelE, RelB and RelBE genes were subcloned(More)
OBJECTIVE To construct the recombinant plasmid containing the outer membrane protein LipL32 gene of Leptospira strain 017 and to study on the cytotoxicity of the expression protein. METHODS By the polymerase chain reaction (PCR), the LipL32 gene was amplified from Leptospira strain 017 genome and cloned into pET32a(+) with enzyme digestion, then used to(More)
AIM To construct the eukaryotic experssion vector of LipL32 gene from Leptospira serovar Lai and express the recombinant plasmid in COS-7 cell. METHODS The LipL32 gene was amplified from Leptospira strain 017 genomic DNA by PCR and cloned into pcDNA3.1, through restriction nuclease enzyme digestion. Then the recombinant plasmid was transformed into E.coli(More)
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