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  • 朱成科, Zhu Cheng-ke, +13 authors Zheng Zong-lin
  • Chemistry
  • 2018 (First Published: 25 February 2018)
  • 趋化因子是一类能够吸引白细胞移行到感染部位的低分子量(8~10 ku)蛋白质,在炎症反应、非特异性免疫反应中具有重要作用。实验克隆了剑尾鱼2个趋化因子基因( CCL 4、 CCL 19)cDNA序列,运用荧光定量PCR技术检测其在健康组织和嗜水气单胞菌感染后肝、脾中的表达情况。结果显示,剑尾鱼 CCL 4和 CCL 19开放阅读框长度分别为294和333Continue Reading
  • 周朝伟, Zhou Chao-wei, +25 authors Zheng Zong-lin
  • Biology
  • 2019 (First Published: 25 June 2019)
  • 为研究crh (Corticotropin-releasing hormone)基因对鱼类摄食的调控作用,首次克隆了银鲫(Carassius auratus gibelio) crh基因cDNA全长序列。运用实时荧光定量PCR(RT-qPCR)技术检测crh基因mRNA在不同组织的表达及餐前、餐后和禁食对其表达的影响。结果显示,银鲫crh基因的cDNA序列全长为920 bp,其中,5'Continue Reading