Zhen-zhen Zhang

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L-Idonate dehydrogenase (L-IdnDH) is the only known enzyme of the tartaric acid (TA) biosynthetic pathway in grape berries. Previous studies on this enzyme only focused on the transcriptional expression of this gene. Here, the antibody against L-IdnDH was prepared by cloning the full-length cDNAs of VvIdnDH from grape berries, highly expressing fusion(More)
Chronic hepatitis B virus (HBV) infection is a major risk factor for liver cirrhosis and hepatocellular carcinoma. Nevertheless, the molecular mechanism of HBV replication remains elusive. SIRT1 is a class III histone deacetylase that is a structure component of the HBV cccDNA minichromosome. In this study, we found by using microarray-based gene expression(More)
AIM To study Hepatitis B virus (HBV) infection and its association with hepatocellular carcinoma (HCC) at the miRNA level. METHODS Three cellular models were used to investigate miRNA expression changes during HBV infection: human HepG2 hepatoblastoma cell line as a model without HBV infection; HepG2 cell line transfected with a 1.3-fold full-length HBV(More)
3-Deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAHPS) is an entry enzyme of the shikimate pathway that connects primary carbohydrate metabolism with the biosynthesis of most secondary metabolites in plants. In the present study, two DAHPS cDNAs were cloned from grape berries (Vitis vinifera) and designated as VvDAHPS-1 and VvDAHPS-2. These two cDNA(More)
Modulation of flavonoid biosynthesis in grape berries has always aroused great attention among researchers. However, little study has been made on the shikimate pathway that guides photo-assimilate flow into flavonoid metabolism. The present study indicated that the treatments of three ultraviolet (UV) wavelengths differentially up-regulated transcriptional(More)
Biofortification is a strategy for overcoming human zinc (Zn) deficiency, especially in rural areas of developing countries. Actually, biofortification by foliar Zn application has been demonstrated at small scale, but not at large scale due to the absence of economic analysis. Therefore, here, we conducted the first cost-effectiveness analysis using the(More)
PURPOSE To characterize the functional role of SIRT6 in hepatocellular carcinoma (HCC). EXPERIMENTAL DESIGN The expression of SIRT6 in 60 paired paraffin-embedded HCC tissues and adjacent nontumoral liver tissues was examined by immunohistochemistry. The expression of SIRT6 in 101 paired frozen HCC tissues and adjacent nontumoral liver tissues was(More)
OBJECTIVE To develop a RNAi approach that specifically targets the HCV IRES sequence by vector-expressed short hairpin RNA (shRNA) in vitro, and to assess the inhibitory effect of the shRNA on reporter gene expression. METHODS Eukaryotic expressing plasmids, pIRES-GFP and p5' UTR-Luc containing GFP or luciferase gene controlled by HCV IRES were(More)
The accumulation of flavan-3-ols in response to ultraviolet (UV) irradiation was investigated in grape berries with emphasis on the expression of three structural genes (VvANR, VvLAR1 and VvLAR2), and three regulatory genes (VvMYB5a, VvMYB5b and VvMYBPA1), and as well as the contents of flavan-3-ols. UV-A irradiation showed a promoting effect on the(More)
The temporal evolution properties of the brain network are crucial for complex brain processes. In this paper, we investigate the differences in the dynamic brain network during resting and visual stimulation states in a task-positive subnetwork, task-negative subnetwork, and whole-brain network. The dynamic brain network is first constructed from human(More)