Zhaoxin Fan

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Thirteen pairs of primers were designed, synthesized and used to clone the whole coding sequences or mature peptide-coding sequences of lipases. Bacteria producing extracellular lipases were enriched for the extraction of total DNAs. Eight fragments with 500–1,200 bp in length were obtained by using touchdown PCR and sequenced. Five of them were found to be(More)
Text clustering plays an important role in data mining and machine learning. After years of development, clustering technology has produced a series of theories and methods. However, in the text clustering of Chinese news, the mainstream LDA method suffers a high time complex. In order to improve the speed, this paper puts forward a new method in which(More)
BACKGROUND To identify the relationship between predialysis pulse wave velocity (PWV), postdialysis PWV during 1 hemodialysis (HD) session, and deaths in maintenance HD patients. METHODS 43 patients were recruited. PWV was measured before and after one HD session and dialysis- related data were recorded. Clinical data such as blood pressure, blood lipids,(More)
OBJECTIVE To evaluate the effect of copper-ion on the proliferation and differentiation of human umbilical vein endothelial cell (HUVEC). METHODS HUVEC were cultured and passaged in vitro. HUVEC were inoculated into 96-well plate with density of 5 x 10(3)/well. All the cells were divided into 3 groups randomly according to different culture mediums: group(More)
Various feeder layers have been extensively applied to support the prolonged growth of human pluripotent stem cells (hPSCs) for in vitro cultures. Among them, mouse embryonic fibroblast (MEF) and mouse fibroblast cell line (SNL) are most commonly used feeder cells for hPSCs culture. However, these feeder layers from animal usually cause immunogenic(More)
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