Zhaoxia Janet Jin

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The present paper reports a highly efficient method of making blunt ends from cohesive ends of double-stranded DNA. Klenow fragment and Pfu DNA polymerases were used to fill in the cohesive ends. Since the transformation efficiency can directly reflect the filling-in efficiency, similar ligation and transformation conditions were used, and the filling-in(More)
Calcium tolerant single ventricular cardiomyocytes were dispersed from adult guinea pig hearts by retrograde perfusion with collagenase solution. More than 50% of the isolated cells retained rod shaped configuration and showed normal electrical activities with resting potentials (RP) at -82 +/- 2 mV and action potential amplitude (APA) at 116 +/- 6 mV. The(More)
AIM To observe the effect of captopril (Cap) on intracellular pH (pHi) in aortic smooth muscle cells (ASMC). METHODS Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pHi was determined using digital image processing method. RESULTS The pHi of untreated SHR and WKY rats were 7.37 +/- 0.29 and 7.19 +/- 0.31,(More)
Guan-fu base A is a new alkaloid first isolated from the tuber of Aconitum coreanum in China. The electrophysiological effects of guan-fu base A were examined on the isolated papillary muscles of guinea pigs by glass-microelectrode technique coupled with microcomputer real-time analysis. Guan-fu base A 50 micrograms/ml had less effect on RP, but markedly(More)
Action potentials of isolated ventricular myocardial fibers of pigs and the electrophysiological actions of dl-demethylcoclaurine (DMC) or higenamine on the porcine myocardial cells were studied by glass microelectrodes. The action potentials of porcine ventricular myocardial fibers were similar to those of other mammalian hearts. Amplitude of the action(More)
The adsorption of extracellular polymeric substances (EPS) from Pseudomonas putida on montmorillonite, kaolinite and goethite was investigated as a function of pH using batch studies coupled with confocal laser scanning microscopy (CLSM) and isothermal titration calorimetry (ITC). Characterization by Raman spectroscopy, X-ray photoelectron spectroscopy(More)