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Seedlings of O. violaceus were cultured on MS media and treated at low temperature. Cold treatment at 5–7 °C for more than 7 days was needed for flower induction of seedlings in vitro originated from germinated seeds. When cultured on MS media supplemented with 2.5 mg l−1 zeatin and 2 mg l−1 gibberellin (GA3), seedlings in vitro did initiate flowers without(More)
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