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Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of CO2 in C4 plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a C4 SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed(More)
Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90 degrees. To probe the(More)
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