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Mutation detection and single-molecule counting using isothermal rolling-circle amplification
Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence ofExpand
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Directly labeled DNA probes using fluorescent nucleotides with different length linkers.
Directly labeled fluorescent DNA probes have been made by nick translation and PCR using dUTP attached to the fluorescent label, Cy3, with different length linkers. With preparation of probes by PCRExpand
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Molecular mechanism controlling the incorporation of fluorescent nucleotides into DNA by PCR.
The efficiency and yield of incorporation of fluorescent nucleotides into DNA by polymerase chain reaction (PCR) have been investigated with linear amplification (PCR with single-stranded templateExpand
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Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD).
Progress has been made in improving the immunohistochemical detection of antigens for imaging and flow cytometry. We report the synthesis of a novel fluorescent horseradish peroxidase substrate,Expand
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Signal Amplification in the Detection of Single-copy DNA and RNA by Enzyme-catalyzed Deposition (CARD) of the Novel Fluorescent Reporter Substrate Cy3.29-Tyramide
We demonstrate that the CAtalyzed Reporter Deposition method (CARD), utilizing the novel fluorescent reporter Cy3.29-tyramide, is successful in the Fluorescent In Situ Hybridization (FISH) detectionExpand
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Directly labeled DNAprobes using fluorescent nucleotides withdifferent length linkers
Directly labeled fluorescent DNAprobeshavebeen madebynick translation andPCRusing dUTPattached tothefluorescent label, Cy3,withdifferent length linkers. Withpreparation ofprobes byPCRwefind thatExpand