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Protein phosphatase type 1 and type 2A assays.
A novel spectroscopic titration method for determining the dissociation constant and stoichiometry of protein-ligand complex.
We offer a new titration protocol for determining the dissociation constant and binding stoichiometry of protein-ligand complex, detectable by spectroscopic methods. This approach neither is limitedExpand
Mechanistic investigation of medium-chain fatty acyl-CoA dehydrogenase utilizing 3-indolepropionyl/acryloyl-CoA as chromophoric substrate analogues.
The CoA derivative 3-indolepropionyl-CoA (IPCoA) serves as a competent pseudosubstrate for the medium-chain fatty acyl-CoA dehydrogenase (MCAD)-catalyzed reaction. The reaction productExpand
Kinetic evaluation of substrate-dependent origin of the lag phase in soybean lipoxygenase-1 catalyzed reactions.
We have measured, under identical conditions, the time courses for the native lipoxygenase (Fe2+ form)-catalyzed conversion of linoleic acid into 13-hydroperoxy-9,11-octadecadienoic acid (HPOD) andExpand
A graphical method for determining the number of essential sites in enzymes with multiple binding sites for a ligand.
We offer a novel graphical method for determining the number of essential sites in enzymes that contain multiple binding sites for a ligand. This method is applicable both to monomeric enzymesExpand
A continuous spectrophotometric assay for protein phosphatases.
A continuous spectrophotometric assay for the determination of protein phosphatase activity is presented. The assay incorporates the coupled enzyme system of Webb (M. R. Webb, 1992, Proc. Natl. Acad.Expand
Stimulation of phosphorylase phosphatase activity of protein phosphatase 2A1 by protamine is ionic strength dependent and involves interaction of protamine with both substrate and enzyme.
The effects of protamine on the phosphorylase phosphatase activity of porcine cardiac protein phosphatase 2A1 (PP2A1) were complex and ionic strength dependent. Under ionic strength conditions thatExpand
Determination and purification of metallothioneins by high performance liquid chromatography.
A rapid, reproducible and sensitive high performance liquid chromatography (HPLC) method for the determination and purification of metallothionein-I (MT-I) and metallothionein-II (MT-II) in mouse andExpand
[Isolation and elucidation of a new minor saponin from the leaves of Panax ginseng C.A. Meyer].
The structure of a new minor saponin isolated from the leaves of Panax ginseng C. A. Meyer was elucidated on the basis of chemical evidences and spectral data. The saponin was named as ginsenoside-La.
A study on photocatalytic activity of micro-arc oxidation TiO2 films and Ag+/MAO-TiO2 composite films
Abstract First, micro-arc oxidation (MAO) TiO 2 films have been prepared on pure titanium in a phosphate-based electrolyte, and then the Ag + /MAO-TiO 2 composite films have been fabricated by Ag +Expand
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