• Publications
  • Influence
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
  • Z. Sváb, P. Maliga
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 February 1993
We report here a 100-fold increased frequency of plastid transformation in tobacco by selection for a chimeric aadA gene encoding aminoglycoside 3"-adenylyltransferase, as compared with that obtainedExpand
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The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
The newpPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322bom site for mobilizationExpand
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Stable transformation of plastids in higher plants.
Stable genetic transformation of the plastid genome is reported in a higher plant, Nicotiana tabacum. Plastid transformation was obtained after bombardment of leaves with tungsten particles coatedExpand
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Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes
The plastid genomes of several plants contain homologues, termed ndh genes, of genes encoding subunits of the NADH:ubiquinone oxidoreductase or complex I of mitochondria and eubacteria. TheExpand
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Expression of tetanus toxin Fragment C in tobacco chloroplasts.
Fragment C (TetC) is a non-toxic 47 kDa polypeptide fragment of tetanus toxin that can be used as a subunit vaccine against tetanus. Expression of TetC in Escherichia coli and yeast was dependent onExpand
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Plastid Transformation in Lesquerella Fendleri, an Oilseed Brassicacea
A plastid transformation protocol was developed for Lesquerella fendleri, a species with a high capacity for plant regeneration in tissue culture. Transformation vector pZS391B carried an aadA16gfpExpand
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Exceptional transmission of plastids and mitochondria from the transplastomic pollen parent and its impact on transgene containment
  • Z. Sváb, P. Maliga
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences
  • 24 April 2007
Plastids in Nicotiana tabacum are normally transmitted to the progeny by the maternal parent only. However, low-frequency paternal plastid transmission has been reported in crosses involving parentsExpand
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Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.
We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated withExpand
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Efficient targeting of foreign genes into the tobacco plastid genome.
The pPRV plasmids are vectors for targeted insertion of foreign genes into the tobacco plastid genome (ptDNA). The vectors are based on the pUC119 plasmid which replicates in E. coli but not inExpand
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Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system.
Incorporation of a selectable marker gene during transformation is essential to obtain transformed plastids. However, once transformation is accomplished, having the marker gene becomes undesirable.Expand
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