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[DNA sequencing by hybridization with oligonucleotides immobilized in a gel. Chemical ligation as a method of expanding the prospects for the method].
It was established that modified oligonucleotides which have a phosphoester bond between the phosphate group and the non-nucleotide substituent are more labile than the corresponding compounds with a phosphoamide bond. Expand
Advanced Organic Chemistry of Nucleic Acids
Structure of nucleotides properties of nucleotides primary structure of nucleic acids determination of the primary structure of nucleic acids conformation of nucleic acid components - macromolecularExpand
Modification of Oligo (Poly) Nucleotide Phosphomonoester Groups in Aqueous Solutions
Abstract Selective modification of oligo (poly) nucleotide phosphomonoester groups in an aqueous medium by N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide in the presence of various nucleophilicExpand
Synthetic nucleotide-peptides.
  • Z. Shabarova
  • Biology, Medicine
  • Progress in nucleic acid research and molecular…
  • 1970
It is concluded that the steric closeness of such synthetic “enzyme-substrate complexes” may give rise to formation of new internucleotide linkages, and one may hope that this principle may become a basic one for the self-assembly of oligonucleotides to form double-stranded polynucleotide. Expand
Oligonucleotide circularization by template-directed chemical ligation.
An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed, and a novel idea, consisting of the incorporation of non-nucleotide insertions in the precursor oligomer, may render this method universal and highly effective. Expand
Physico-chemical and biological properties of antisense phosphodiester oligonucleotides with various secondary structures.
Oligonucleotides having hairpin, looped and snail-like structure are found to be much more stable to nuclease degradation in different biological media and inside cells than the linear ones. Expand
The cysteine conserved among DNA cytosine methylases is required for methyl transfer, but not for specific DNA binding.
It is shown that a DNA substrate for M. EcoRII in which the target cytosine is replaced by 5-fluorocytosine are a mechanism-based inhibitor of the enzyme and that this modified substrate does not form irreversible complexes with the mutants. Expand
Constructing DNA by polymerase recombination
Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmidExpand
Site-directed modification of DNA duplexes by chemical ligation.
The efficiency of chemical ligation method have been demonstrated by assembling a number of DNA duplexes with modified sugar phosphate backbone by performing condensation on a tetradecanucleotide template of hexa(penta)- and undecan nucleoside residue using water-soluble carbodiimide as a condensing agent. Expand
Crosslinking of double-stranded oligonucleotides containing O-methyl-substituted pyrophosphate groups to the HNF1 transcription factor in nuclear cell extract.
Results indicate that DNA duplexes carrying substituted pyrophosphate internucleotide groups can specifically bind and crosslink with DNA-binding proteins, especially transcription factors in crude preparations and could constitute a potential tool to control the expression of disease-causing genes. Expand