• Publications
  • Influence
Transient ribosomal attenuation coordinates protein synthesis and co-translational folding
It is proposed that local discontinuous translation temporally separates the translation of segments of the peptide chain and actively coordinates their co-translational folding in response to ribosome-mediated translational attenuation. Expand
Emerging roles of tRNA in adaptive translation, signalling dynamics and disease
tRNAs, nexus molecules between mRNAs and proteins, have a central role in translation. Recent discoveries have revealed unprecedented complexity of tRNA biosynthesis, modification patterns,Expand
Secondary Structure across the Bacterial Transcriptome Reveals Versatile Roles in mRNA Regulation and Function
This work determines for the first time the E. coli RNA structurome, highlighting the contribution of mRNA secondary structure as a direct effector of a variety of processes, including translation and mRNA degradation. Expand
Efficient translation initiation dictates codon usage at gene start
It is shown that in bacteria selection favours codons that reduce mRNA folding around the translation start, regardless of whether these codons are frequent or rare, and that primarily mRNA structure, and not codon usage, at the beginning of genes determines the translation rate. Expand
Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling.
Aggregation in vivo displayed a concentration-dependent apparent lag time similar to observations of protein aggregation in purified in vitro model systems. Expand
Generic Algorithm to Predict the Speed of Translational Elongation: Implications for Protein Biogenesis
Using the complete genomes of Escherichia coli and Bacillus subtilis, it is shown that stretches of codons pairing to minor tRNAs form putative sites to locally attenuate translation, suggesting a concerted selective pressure on the codon selection and species-specific tRNA abundance in these regions. Expand
Unusual signal peptide directs penicillin amidase from Escherichia coli to the Tat translocation machinery.
Mutational studies have shown that the hydrophobic core region acts in synergism with the positive charged N-terminal part of the signal peptide as a Tat recognition signal and contributes to the efficient Tat targeting of the pre-pro-penicillin amidase. Expand
Isolation and partial characterisation of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus.
Evidence was presented that substrate induction was a major regulatory mechanism and the keratinase biosynthesis was not completely repressed by addition of other carbon (glucose) and nitrogen (NH4C1) sources. Expand
Inhibition of protein aggregation in vitro and in vivo by a natural osmoprotectant
The osmolyte proline may be protective against biomedically important protein aggregates that are hallmarks of several late-onset neurodegenerative diseases including Huntington's, Alzheimer's, and Parkinson’s. Expand
Dynamic m6A methylation facilitates mRNA triaging to stress granules
It is discovered that in response to oxidative stress, transcripts are additionally m6A modified in their 5′ vicinity, and this methylation pattern provides a selective mechanism for triaging mRNAs from the translatable pool to stress-induced stress granules. Expand