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Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR.
In competitive RNA-PCR studies, contaminating DNA can produce incorrect results because of its potential to act as a second competitor. Preliminary studies using published methods for DNase I… Expand
Expression of cytochromes P450 in human breast tissue and tumors.
- Z. Huang, M. Fasco, H. Figge, K. Keyomarsi, L. S. Kaminsky
- Biology, Medicine
- Drug metabolism and disposition: the biological…
- 1 August 1996
In an effort to determine which members of the cytochrome P450 (CYP) superfamily are expressed in human breast tissue and tumors, RNA-polymerase chain reaction studies have been undertaken. Detection… Expand
GluR3 flip and flop: differences in channel opening kinetics.
Ample evidence from earlier studies of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, GluR3 included, suggests that alternative splicing not only enriches AMPA receptor… Expand
De novo assembly of a chromosome‐level reference genome of red‐spotted grouper (Epinephelus akaara) using nanopore sequencing and Hi‐C
The red‐spotted grouper Epinephelus akaara (E. akaara) is one of the most economically important marine fish in China, Japan and South‐East Asia and is a threatened species. The species is also… Expand
Molecular markers linked to Bn;rf: a recessive epistatic inhibitor gene of recessive genic male sterility in Brassica napus L.
Abstract7–7365AB is a recessive genic male sterile (RGMS) two-type line, which can be applied in a three-line system with the interim-maintainer, 7–7365C. Fertility of this system is controlled by… Expand
Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety.
- M. Li, N. Lin, +4 authors B. Wang
- Chemistry, Medicine
- Journal of the American Chemical Society
- 3 September 2008
The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have… Expand
Ester and amide derivatives of E64c as inhibitors of platelet calpains.
Ester and amide derivatives of E64c, (+)-(2S,3S)-3-[[(S)-3-methyl-1- [(3-methylbutyl)carbamoyl]butyl]carbamoyl]-2-oxiranecarboxylic acid, an inhibitor of calpains, were synthesized and tested for… Expand
Effect of cyclodextrin and membrane lipid structure upon cyclodextrin-lipid interaction.
Methyl-β-cyclodextrin (MβCD) can be used to exchange membrane lipids between different vesicles in order to prepare model membrane vesicles with lipid asymmetry. To help define what factors influence… Expand
The Full-length Unprocessed Hedgehog Protein Is an Active Signaling Molecule*
- R. Tokhunts, S. Singh, +11 authors D. Robbins
- Biology, Medicine
- The Journal of Biological Chemistry
- 17 November 2009
The hedgehog (HH) family of ligands plays an important instructional role in metazoan development. HH proteins are initially produced as ∼45-kDa full-length proteins, which undergo an intramolecular… Expand
One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors
RNA is ideally suited for in vitro evolution experiments, because a single RNA molecule possesses both genotypic (replicable sequence) and phenotypic (selectable shape) properties. Using systematic… Expand