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Combined genome and transcriptome analysis of single disseminated cancer cells from bone marrow of prostate cancer patients reveals unexpected transcriptomes.
Bone is the most frequent site of metastasis in prostate cancer and patients with bone metastases are deemed incurable. Targeting prostate cancer cells that disseminated to the bone marrow before… Expand
Quantitative Measurement of Melanoma Spread in Sentinel Lymph Nodes and Survival
In this study, Klein and colleagues investigated the impact of minimal cancer sentinel lymph node spread and of increasing numbers of disseminated cancer cells on melanoma-specific survival. The… Expand
Reliable Single Cell Array CGH for Clinical Samples
Background Disseminated cancer cells (DCCs) and circulating tumor cells (CTCs) are extremely rare, but comprise the precursors cells of distant metastases or therapy resistant cells. The detailed… Expand
Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during clinical latency
- Melanie Werner-Klein, Ana Grujovic, +30 authors C. Klein
- Biology, Medicine
- Nature communications
- 5 October 2020
Although thousands of breast cancer cells disseminate and home to bone marrow until primary surgery, usually less than a handful will succeed in establishing manifest metastases months to years… Expand
HER2 FISH results in breast cancers with increased CEN17 signals using alternative chromosome 17 probes – reclassifying cases in the equivocal category
- Maria-Anna Holzschuh, Z. Czyż, Sven Hauke, E. Inwald, Bernhard Polzer, G. Brockhoff
- Biology, Medicine
- 1 October 2017
HER2 testing of invasive breast cancer by in‐situ hybridization guides therapy decisions. Probing HER2 and centromere of chromosome 17 (cen17) simultaneously is supposed to reveal both a potential… Expand
Molecular Profiling of Single Sca-1+/CD34+,− Cells—The Putative Murine Lung Stem Cells
Murine bronchioalveolar stem cells play a key role in pulmonary epithelial maintenance and repair but their molecular profile is poorly described so far. In this study, we used antibodies directed… Expand
Principles of Whole-Genome Amplification.
Modern molecular biology relies on large amounts of high-quality genomic DNA. However, in a number of clinical or biological applications this requirement cannot be met, as starting material is… Expand
Deterministic Whole-Genome Amplification of Single Cells.
This chapter describes a single-cell whole genome amplification method (WGA) that has been originally published under the name "Single Cell Comparative Genomic Hybridization (SCOMP)" (Klein et al.,… Expand
Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1™.
Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a… Expand