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Flexible use of nuclear import pathways by HIV-1.
A macaque model of HIV-1 infection
- T. Hatziioannou, Z. Ambrose, P. Bieniasz
- BiologyProceedings of the National Academy of Sciences
- 17 March 2009
Simian-tropic (st)HIV-1 strains that differ from HIV-1 only in the vif gene are constructed that are capable of high levels of replication in vitro in pig-tailed macaque lymphocytes and demonstrate the potential utility in a chemoprophylaxis experiment.
Human Immunodeficiency Virus Type 1 Capsid Mutation N74D Alters Cyclophilin A Dependence and Impairs Macrophage Infection
Compared to wild-type (WT), N74D HIV-1 is more sensitive to cyclosporine, has increased sensitivity to nevirapine, and is impaired in macrophage infection prior to reverse transcription, which suggests a difference in the N 74D reverse transcription complex that manifests early after infection and prior to interaction with the nuclear pore.
HIV/AIDS: in search of an animal model.
HIV-1 uncoating: connection to nuclear entry and regulation by host proteins.
Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay
A novel RNA accessibility-based assay that detects an early event in HIV-1 uncoating and can be used to further define this process is established.
Rilpivirine and Doravirine Have Complementary Efficacies Against NNRTI-Resistant HIV-1 Mutants
- Steven J Smith, G. Pauly, S. Hughes
- BiologyJournal of acquired immune deficiency syndromes
- 15 August 2016
It is shown that DOR is susceptible to a number of well-known NNRTI resistance mutations and an understanding of the mutational susceptibilities and binding interactions of NN RTIs with RT could be used to develop pairs of compounds with non-overlapping mutATIONAL susceptibilities.
Genetic Diversity of Simian Immunodeficiency Virus Encoding HIV-1 Reverse Transcriptase Persists in Macaques despite Antiretroviral Therapy
Findings indicate that viral diversity does not decrease with suppressive ART, that ongoing replication occurs with viremias >15 copies/ml, and that in this macaque model of ART drug resistance likely emerges as a result of incomplete suppression and preexisting drug resistance mutations.
The nucleoside analogs 4'C-methyl thymidine and 4'C-ethyl thymidine block DNA synthesis by wild-type HIV-1 RT and excision proficient NRTI resistant RT variants.
AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography
- Xiaofeng Fu, J. Ning, Z. Zhong, Z. Ambrose, Simon Charles Watkins, Peijun Zhang
- BiologyScientific Reports
- 1 December 2019
This AutoCLEM workflow significantly accelerates the correlation efficiency between live-cell fluorescence imaging and cryoEM/ET structural analysis, as demonstrated by visualizing human immunodeficiency virus type 1 (HIV-1) interacting with host cells.