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Small RNAs GlmY and GlmZ compose a cascade that feedback-regulates synthesis of enzyme GlmS in Enterobacteriaceae. Here, we analyzed the transcriptional regulation of glmY/glmZ from Yersinia pseudotuberculosis, Salmonella typhimurium and Escherichia coli, as representatives for other enterobacterial species, which exhibit similar promoter architectures. The(More)
In E. coli, small RNA GlmZ activates the glmS mRNA by base-pairing in an Hfq dependent manner. When not required, GlmZ is bound by adaptor protein RapZ and recruited to RNase E, which cleaves GlmZ in its base-pairing sequence. Small RNA GlmY counteracts cleavage of GlmZ by sequestration of RapZ. Although both sRNAs are highly homologous, only GlmZ(More)
Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults and has a very poor prognosis. 1 Part of the challenge in treating GBM is related to its localization within the naturally immunosuppressive central nervous system (CNS), which is anatomically-protected by a blood brain barrier and devoid of the traditional lymphatic structure.(More)
Formation of glucosamine-6-phosphate (GlcN6P) by enzyme GlcN6P synthase (GlmS) represents the first step in bacterial cell envelope synthesis. In Escherichia coli, expression of glmS is controlled by small RNAs (sRNAs) GlmY and GlmZ. GlmZ activates the glmS mRNA by base-pairing. When not required, GlmZ is bound by adapter protein RapZ and recruited to(More)
Signal perception and transduction is a key mechanism ensuring bacterial adaptation to environmental changes. By connecting two-component signal transduction systems and small regulatory RNAs, multiple stimuli can be integrated to fine-tune gene expression. The unique properties of small RNAs extend the flexibility, timing, and dynamics of two-component(More)
Ménage à trois: post-transcriptional control of the key enzyme for cell envelope synthesis by a base-pairing small RNA, an RNase adaptor protein , and a small RNA mimic. ¯ Die Kaskade aus den sRNAs GlmY und GlmZ und dem Adapterprotein RapZ steu-ert die Expression der Glukosamin-6-phosphat-Synthase (GlmS) in Escherichia coli. Ist viel Glukosamin-6-phosphat(More)
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