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Mariner-like elements are widespread eukaryotic transposons, but Mos-1 is the only natural element that is known to be active. Little is known about the biochemistry of mariner transposition. The first step in the process is the binding of the transposase to the 5′ and 3′ inverted terminal repeats (ITRs) of the element. Using the 3′ ITR of the element, we(More)
The two inverted terminal repeats (ITRs) flanking the Mos-1 mariner element differ in sequence at four positions. Gel retardation experiments indicated that each of these differences has a significant impact on the quality of the interaction between the ITR and the Mos-1 transposase. We showed that the transposase binds to the 3′ ITR better than to the 5′(More)
The nucleocytoplasmic large DNA viruses (NCLDVs) comprise a monophyletic group of viruses that infect animals and diverse unicellular eukaryotes. The NCLDV group includes the families Poxviridae, Asfarviridae, Iridoviridae, Ascoviridae, Phycodnaviridae, Mimiviridae and the proposed family “Marseilleviridae”. The family Mimiviridae includes the largest known(More)
Large quantities of satellite DNA families (15%-25% of the genome) were found in the DNA of two species of parasitic wasps, Diadromus pulchellus and Eupelmus vuilleti. In both species the satellite DNA was found to consist wholly or largely of a single family unique to that species. Several clones of each family were obtained and sequenced. Palindromes in(More)
Ascoviruses (family Ascoviridae) are double-stranded DNA viruses with circular genomes that attack lepidopterans, where they produce large, enveloped virions, 150 by 400 nm, and cause a chronic, fatal disease with a cytopathology resembling that of apoptosis. After infection, host cell DNA is degraded, the nucleus fragments, and the cell then cleaves into(More)
The Diadromus pulchellus ascovirus (DpAV) has been isolated from laboratory strains of Diadromus pulchellus and in natural wild populations collected from the Antibes locality (southern France). The DpAV genome was found in the cells of the head, thorax and abdomen of this hymenopteran wasp. DpAV virions are present in the female genitalia and are(More)
Internal fragments of the putative transposase gene of mariner-like elements (MLEs) were amplified from human, mouse, rat, chinese hamster, sheep and bovine genomic DNAs by polymerase chain reaction (PCR). The sequences identified in human, ovine and bovine genomes correspond to ancient degenerate transposons. Screening mammalian sequence libraries(More)
Copies of mariner-like element (MLE) transposons in two species, the bumble bee, Bombus terrestris, and the ant, Myrmica ruginodis, were sequenced. The full-sized elements are 1250 bp long in both species and include 28-bp inverted terminal repeats. The five copies sequenced were approximately 75% similar to a mariner element (peach) of Drosophila(More)
The mobility of transposable elements via a cut-and-paste mechanism depends on the elaboration of a nucleoprotein complex known as the synaptic complex. We show here that the Mos1 synaptic complex consists of the two inverted terminal repeats of the element brought together by a transposase tetramer and is designated paired-end complex 2 (PEC2). The(More)
A new member of the family Ascoviridae, Diadromus pulchellus ascovirus (DpAV), has been found in the lepidopteran nymphs of Acrolepiopsis assectella parasitized by the hymenopteran wasp Diadromus pulchellus. Virions have the standard features of the ascovirus group; each particle is about 220 nm long and 150 nm wide. They are multilayered, with two clear(More)