Yuya Shigenobu

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Nori, a marine red alga, is one of the most profitable mariculture crops in the world. However, the biological properties of this macroalga are poorly understood at the molecular level. In this study, we determined the draft genome sequence of susabi-nori (Pyropia yezoensis) using next-generation sequencing platforms. For sequencing, thalli of P. yezoensis(More)
Tunas are migratory fishes in offshore habitats and top predators with unique features. Despite their ecological importance and high market values, the open-ocean lifestyle of tuna, in which effective sensing systems such as color vision are required for capture of prey, has been poorly understood. To elucidate the genetic and evolutionary basis of optic(More)
To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were(More)
The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite(More)
In recent years, massive sequencing approaches have allowed us to determine genomic structures of various organisms rapidly, raising novel applicability of the high-throughput sequence data obtained to various fields of biological studies. We present here a pipeline to search for microsatellite DNA and design PCR primers encompassing the microsatellites on(More)
To clarify the level of contamination with radioactive cesium (radiocesium) discharged from Fukushima Dai-ichi Nuclear Power Plant (FDNPP), three fish species caught in the main harbor of FDNPP were subjected to γ-ray analysis. The concentration of radiocesium in muscle differed among individual fish, even those of similar size of the same species, and(More)
Heteroplasmic nucleotide polymorphisms are rarely observed in wild animal mitochondrial DNA. The occurrence of such site heteroplasmy is expected to be extremely rare at nonsynonymous sites where the number of nucleotide substitutions per site is low due to functional constraints. This report deals with nonsynonymous mitochondrial heteroplasmy from two wild(More)
The Fukushima Dai-ichi nuclear power plant (FNPP) accident that followed the Great East Japan Earthquake in 2011 resulted in the release of enormous quantities of anthropogenic radionuclides, especially radioactive cesium (134Cs and 137Cs) into the ocean off the east coast of Japan. FNPP-derived radioactive Cs may have consequently accumulated within marine(More)
Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japana; Department of Marine Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima, Japanb; Research Center for Fisheries Oceanography and Marine Ecosystem, National Research Institute of(More)
Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japana; Department of Marine Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hirosima, Japanb; Research Center for Fisheries Oceanography and Marine Ecosystem, National Research Institute of(More)