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Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment1[OPEN]
Hydrogen production catalyzed by the extremely anaerobic enzyme [FeFe]-hydrogenase in air-grown microalgae reports on microoxic niches within the thylakoid stroma. Photosynthetic hydrogen productionExpand
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Green Algal Hydrogenase Activity Is Outcompeted by Carbon Fixation before Inactivation by Oxygen Takes Place1
In the transition from dark anaerobiosis to light, oxygen deactivates the hydrogenase pool, but only after carbon fixation outcompetes hydrogen production for electrons. Photoproduction of hydrogenExpand
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The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance
BackgroundHydrogen photo-production in green algae, catalyzed by the enzyme [FeFe]-hydrogenase (HydA), is considered a promising source of renewable clean energy. Yet, a significant increase inExpand
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Overcoming the expression barrier of the ferredoxin‑hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output
Abstract While the prospect of producing hydrogen from photosynthetic microalgae has long been described as one of the promising directions towards achieving a renewable fuel source, currentExpand
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Rewiring photosynthesis: a photosystem I-hydrogenase chimera that makes H2in vivo
Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As aExpand
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Juggling Lightning: How Chlorella ohadii handles extreme energy inputs without damage.
The green alga Chlorella ohadii was isolated from a desert biological soil crust, one of the harshest environments on Earth. When grown under optimal laboratory settings it shows the fastest growthExpand
Bi-directional electron transfer between H2 and NADPH mitigates the response to light fluctuations in green algae
The metabolism of green algae has been the focus of much research over the last century. These photosynthetic organisms can thrive under various conditions and adapt quickly to changing environmentsExpand
Bi-directional electron transfer between H2 and NADPH mitigates light fluctuation responses in green algae.
The metabolism of green algae has been the focus of much research over the last century. These photosynthetic organisms can thrive under various conditions and adapt quickly to changing environmentsExpand
The Mehler reaction site is the Phylloquinone within Photosystem I
Photosynthesis is a vital process, responsible for fixing carbon dioxide, and producing most of the organic matter on the planet. However, photosynthesis has some inherent limitations in utilizingExpand