Yutian Gan

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One direct route for the discovery of therapeutic human monoclonal antibodies (mAbs) involves the isolation of peripheral B cells from survivors/sero-positive individuals after exposure to an infectious reagent or disease etiology, followed by single-cell sequencing or hybridoma generation. Peripheral B cells, however, are not always easy to obtain and(More)
811 To determine an appropriate scoring threshold for this study, we conducted a false positive rate (FPR) analysis to assess the likelihood of random spectral matches to the given protein sequence (Supplementary Fig. 1). Scores of ≥100 were considered unlikely to have arisen by random chance (FPR < 10-5). The software is freely available for download(More)
ISWI chromatin remodelers mobilize nucleosomes to control DNA accessibility. Complexes isolated to date pair one of six regulatory subunits with one of two highly similar ATPases. However, we find that each endogenously expressed ATPase co-purifies with every regulatory subunit, substantially increasing the diversity of ISWI complexes, and we additionally(More)
BACKGROUND Protein secretion to the periplasm of Escherichia coli offers an attractive route for producing heterologous proteins including antibodies. In this approach, a signal peptide is fused to the N-terminus of the heterologous protein. The signal peptide mediates translocation of the heterologous protein from the cytoplasm to the periplasm and is(More)
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