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Symbiotic Chlorella F36-ZK isolated from Paramecium bursaria F36 has constitutive amino acid transport systems, whereas free-living Chlorella does not. We found that in symbiotic algae, the rate of serine (Ser) uptake increased in the presence of glucose (Glc) and non-metabolisable analogues, whilst incorporation of Ser into protein was not affected. The(More)
Analyses of amino acid transport systems in JapaneseParamecium symbiont F36-ZK were performed using14C-amino acids. Kinetic analyses of amino acid uptake and competitive experiments revealed three transport systems; a basic amino acid transport system, which catalyzed transport of L-Arg and L-Lys, a general amino acid transport system, which had broad(More)
Ionophores CCCP (100 μM) 5.11 ± 1.56 1.26 ± 0.48 3.20 ± 0.17 Valinomycin (10 μM) 98.0 ± 5.90 100 ± 14.2 100 ± 12.2 Nigericin (10 μM) 133 ± 7.82 123 ± 11.6 125 ± 9.09 Valinomycin/Nigericin (each 10 μM) 127 ± 8.36 111 ± 6.69 109 ± 1.99 Inhibitors of ATP synthesis NaN3 (1 mM) 17.6 ± 0.82 4.87 ± 0.87 4.70 ± 0.74 DCCD (100 μM) 87.9 ± 3.29 100 ± 3.12 90.2 ± 4.96(More)
We observed high-resolution photoassociation spectra of laser-cooled ytterbium (Yb) atoms in the spin-forbidden 1S0 - 3P1 intercombination line. The rovibrational levels in the 0u+ state were measured for red detunings of the photoassociation laser ranging from 2.9 MHz to 1.97 GHz with respect to the atomic resonance. The rotational splitting of the(More)
We performed an environmental study of viruses infecting the symbiotic single-celled algae of Paramecium bursaria (Paramecium bursaria Chlorella virus, PBCV) in Lake Biwa, the largest lake in Japan. The viruses detected were all Chlorella variabilis virus (CvV = NC64A virus). One of them, designated CvV-BW1, was subjected to further characterization.(More)
The green ciliate Paramecium bursaria contains several hundred symbiotic Chlorella species. We previously reported that symbiotic algal carbon fixation is enhanced by P. bursaria extracts and that the enhancing factor is a heat-stable, low-molecular-weight, water-soluble compound. To identify the factor, further experiments were carried out. The enhancing(More)
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