Yutaka Kashihara

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1. The amount of calcitonin gene-related peptide (CGRP) released from the isolated rat soleus muscle was measured by enzyme immunoassay. 2. When the soleus muscle was exposed to a solution containing high K+ (20-100 mM) in the presence of tetrodotoxin, the amount of CGRP released into the bathing medium increased with an increase in the K+ concentration. 3.(More)
Recovery from destruction by sodium deoxycholate (DOC) was studied with the receptor membrane of the blowfly, Phormia regina. The recovery can be divided into two processes, colchicine dependent and colchicine independent. The colchicine-dependent process was completely depressed by pretreatment with colchicine at 5 mM for 2 min (partially at 0.1 mM for 10(More)
1. Motoneurone death induced by axotomy in the rat was studied following section of the medial gastrocnemius nerve near the muscle 4 days after birth. 2. The maximum twitch tension of the medial gastrocnemius muscle achieved by motor reinnervation after section of its nerve was about 70% of that measured on the contralateral, intact side. 3. The number of(More)
Calcitonin gene-related peptide (CGRP) has been found in both sensory and motor neurons. It has been suggested that CGRP is transported from neuron cell bodies to their terminals, where it may act as an anterograde trophic factor. However, it is not known how fast CGRP is transported or whether CGRP found in the innervated target organ indeed originated in(More)
Sensory neurons with small diameters (A delta and C cells) are known to be responsive to exogenous NGF even at postnatal stages. We have examined whether large Group Ia sensory neurons (A alpha cells) arising from muscle spindles are also responsive to NGF in neonatal rats. For this purpose, monosynaptic excitatory postsynaptic potentials (EPSPs) were(More)
Calcitonin gene-related peptide (CGRP) in skeletal muscle is of neural origin. After degeneration of sensory fibers induced by removal of the lumbar dorsal root ganglia (DRG), the CGRP content in the rat soleus muscle was reduced to about 5% of the normal level. In contrast, degeneration of motor fibers induced by sectioning of the lumbar ventral roots(More)
Homeobox-containing genes are associated with the control of various stages in embryogenesis. LIM-homeodomain genes have been implicated in the control of differentiation of specific cell types in both neuronal and non-neuronal tissues, yet there have been few studies comparing the expression domains of these genes in the developing brain of a single(More)
Calcitonin gene-related peptide (CGRP) is present in both motor and sensory neurons and transported in the somatofugal direction. CGRP levels in sensory neurons are assumed to be regulated by NGF supplied from their peripheral targets. In cultured sensory neurons, however, a basal level of CGRP persists even without NGF. This suggests that some additional(More)
By reverse-transcription polymerase chain reaction method, we isolated a novel murine LIM-homeodomain gene, L3. In situ hybridization analyses revealed that L3 mRNA was localized to the ventral telencephalon and the mesenchyme surrounding the oral cavity of mouse embryo, suggesting that L3 may be involved in the region-specific differentiation of these(More)
In the present study, we isolated five murine LIM-homeodomain (LH) genes including a novel gene designated as L3 which is expressed specifically in the medial ganglionic eminence of the embryonic brain and the mesenchyme surrounding the oral cavity. The comparison of the expression domains in the embryonic forebrain using in situ hybridization(More)