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Strategies for adding sucrose to stationary phase cultures of Sclerotium glucanicum to improve scleroglucan production were examined. Particular attention was paid to the effect of sucrose supplementation on the formation of the toxic by-product, oxalic acid. The rate of addition of sucrose was found to markedly influence the outcome of the process, with(More)
Velvet antler (VA) is used in traditional Chinese medicine to treat a wide range of ailments including the enhancement of wound healing. A 3.2 kDa recombinant polypeptide of VA from sika deer was purified and compared to native polypeptides stimulation growth of NIH3T3 cells. Both stimulated growth in a dose-dependent manner (10–100 μg/ml). To study its(More)
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