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The gene for the D-mandelate dehydrogenase (D-ManDH) of Enterococcus faecalis IAM10071 was isolated by means of an activity staining procedure and PCR and expressed in Escherichia coli cells. The recombinant enzyme exhibited high catalytic activity toward various 2-ketoacid substrates with bulky hydrophobic side chains, particularly C3-branched substrates(More)
Whereas ionizing radiation (Ir) instantaneously causes the formation of water radiolysis products that contain some reactive oxygen species (ROS), ROS are also suggested to be released from biological sources in irradiated cells. It is now becoming clear that these ROS generated secondarily after Ir have a variety of biological roles. Although mitochondria(More)
AIM We report on a rare case of appendiceal primary neuroendocrine carcinoma (NEC) and discuss three cases previously described. CASE REPORT A 58-year-old woman presented with acute abdominal pain and a low-grade fever. She was diagnosed with acute appendicitis and underwent laparoscopic appendectomy. Pathological examination of the resected specimen(More)
In this paper, we propose a novel approach to positioning mobile phone users in a crowd of people. We assume such a situation that people in specific space (e.g., an exhibition hall) have mobile phones and they periodically probe proximity between the surrounding users via short-range wireless communication (e.g., Bluetooth). We also assume that laser range(More)
Nanodiscs are phospholipid-protein complexes which are relevant to nascent high-density lipoprotein and are applicable as a drug carrier and a tool to immobilize membrane proteins. We evaluated the structure and dynamics of the nanoparticles consisting of dimyristoylphosphatidylcholine (DMPC) and apolipoprotein A-I (apoA-I) with small-angle neutron(More)
Urinary nephrin is a potential non-invasive biomarker of disease. To date, however, most studies of urinary nephrin have been conducted in animal models of diabetic nephropathy, and correlations between urinary nephrin-to-creatinine ratio and other parameters have yet to be evaluated in animal models or patients of kidney disease with podocyte dysfunction.(More)
BACKGROUND AND OBJECTIVE Sitting posture may often place large burden on trunk muscles, while trunk muscle activities in the sitting posture have not been well clarified. In this study, a difference in trunk muscle activity between two kinds of sitting postures was evaluated, focusing on low back pain induced by posture holding. MATERIAL AND METHODS An(More)
In this study, a high-throughput and high-sensitivity profiling system for β-cryptoxanthin (βCX) and β-cryptoxanthin fatty acid ester (βCXFA) was constructed by supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry (QqQMS). βCX and nine βCXFAs were successfully separated within 20 min using a column packed with(More)