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The spike protein of SARS-CoV — a target for vaccine and therapeutic development
Recent advances in the development of vaccines and therapeutics based on the S protein are highlighted, which plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. Expand
Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine
The receptor-binding domain (RBD) in SARS-CoV-2 S protein was identified and it was found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors and could block the binding and, hence, attachment of SARs-Cov-2 RBD and SARS -CoV RBD to ACE2-expressing cells, thus inhibiting their infection to host cells. Expand
Molecular Mechanism for Antibody-Dependent Enhancement of Coronavirus Entry
A neutralizing monoclonal antibody, which targets the receptor-binding domain of Middle East respiratory syndrome (MERS) coronavirus spike, mediates viral entry using pseudovirus entry and biochemical assays, and results showed that MAb binds to the virus surface spike, allowing it to undergo conformational changes and become prone to proteolytic activation. Expand
Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains Multiple Conformation-Dependent Epitopes that Induce Highly Potent Neutralizing Antibodies
The RBD of SARS S protein contains multiple conformational epitopes capable of inducing potent neutralizing Ab responses, and is an important target site for developing vaccines and immunotherapeutics. Expand
Human intestinal tract serves as an alternative infection route for Middle East respiratory syndrome coronavirus
It is demonstrated that human primary intestinal epithelial cells, small intestine explants, and intestinal organoids were highly susceptible to MERS-CoV and can sustain robust viral replication and suggest that the human intestinal tract may serve as an alternative infection route for MERS -CoV. Expand
Receptor-binding domain of SARS-CoV spike protein induces highly potent neutralizing antibodies: implication for developing subunit vaccine
It is shown that a recombinant fusion protein (designated RBD-Fc) containing 193-amino acid RBD and a human IgG1 Fc fragment can inducehighly potent antibody responses in the immunized rabbits and suggests that RBD can induce highly potent neutralizing antibody responses and has potential to be developed as an effective and safe subunit vaccine for prevention of SARS. Expand
MERS-CoV spike protein: a key target for antivirals
This review illustrates MERS-CoV S protein’s structure and function, particularly S1 receptor-binding domain (RBD) and S2 heptad repeat 1 (HR1) as therapeutic targets, and summarizes current advancement on developing anti-MERS- coV therapeutics, focusing on neutralizing monoclonal antibodies (mAbs) and antiviral peptides. Expand
Identification of Immunodominant Sites on the Spike Protein of Severe Acute Respiratory Syndrome (SARS) Coronavirus: Implication for Developing SARS Diagnostics and Vaccines
It is shown that the S protein of SARS-CoV is highly immunogenic during infection and immunizations, and contains five linear immunodominant sites (sites I to V) as determined by Pepscan analysis with a set of synthetic peptides overlapping the entire S protein sequence against the convalescent sera from SARS patients and antisera from small animals immunized with inactivated SARS -CoV. Expand
Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
  • Hongjing Gu, Qi Chen, +29 authors Yusen Zhou
  • Medicine
  • Science
  • 30 July 2020
A mouse model in which a SARS-CoV-2 strain was infectious and could cause an inflammatory response and moderate pneumonia is developed, and a panel of adaptive mutations potentially associated with the increased virulence are revealed. Expand
Blockade of the C5a–C5aR axis alleviates lung damage in hDPP4-transgenic mice infected with MERS-CoV
The data indicate that dysregulated host immune responses contribute to the severe outcome of MERS; excessive complement activation, triggered by MERS-CoV infection, promote such dysregulation; and blockade of the C5a–C5aR axis lead to the decreased tissue damage induced by Mers-Cov infection, as manifested by reduced apoptosis and T cell regeneration in the spleen. Expand