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Mutations in human CEP290 cause cilia-related disorders that range in severity from isolated blindness to perinatal lethality. Here, we describe a Chlamydomonas reinhardtii mutant in which most of the CEP290 gene is deleted. Immunoelectron microscopy indicated that CEP290 is located in the flagellar transition zone in close association with the prominent(More)
Intraflagellar transport (IFT), which is the bidirectional movement of particles within flagella, is required for flagellar assembly. IFT particles are composed of approximately 16 proteins, which are organized into complexes A and B. We have cloned Chlamydomonas reinhardtii and mouse IFT46, and show that IFT46 is a highly conserved complex B protein in(More)
Intraflagellar transport (IFT), the bidirectional movement of particles along flagella, is essential for flagellar assembly. The motor for retrograde IFT in Chlamydomonas is cytoplasmic dynein 1b, which contains the dynein heavy chain DHC1b and the light intermediate chain (LIC) D1bLIC. To investigate a possible role for the LIC in IFT, we identified a(More)
Eukaryotic cilia and flagella are vital sensory and motile organelles. The calcium channel PKD2 mediates sensory perception on cilia and flagella, and defects in this can contribute to ciliopathic diseases. Signaling from Pkd2-dependent Ca²+ rise in the cilium to downstream effectors may require intermediary proteins that are largely unknown. To identify(More)
Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. iv Acknowledgments I would like to thank my graduate advisor Dr. George Witman for his guidance, encouragement, and support. He provided me with endless opportunities to develop my scientific thinking and to communicate my work in writing(More)
The lateral mobility of the vesicular stomatitis virus spike glycoprotein (G protein) and various mutant G proteins produced by site-directed mutagenesis of the G cDNA has been measured. Fluorescence recovery after photobleaching results for the wild type G protein in transfected COS-1 cells yielded a mean diffusion coefficient (D) of 8.5 (+/- 1.3) X(More)
In the plasma membrane of animal cells, many membrane-spanning proteins exhibit lower lateral mobilities than glycosylphosphatidylinositol (GPI)-linked proteins. To determine if the GPI linkage was a major determinant of the high lateral mobility of these proteins, we measured the lateral diffusion of chimeric membrane proteins composed of normally(More)
A membrane fraction denoted N2 upper was isolated from homogenates of rat liver by sucrose gradient centrifugation. This fraction, which was enriched 65-fold over the homogenate in 5'-nucleotidase activity, was used as an immunogen in goats. The antisera obtained contained antibodies to three predominant polypeptides in the N2 upper membrane fraction, as(More)
Traffic flow detection plays an important role in Intelligent Transportation Systems(ITS). Video based traffic flow detection system is the most widely used strategy in ITS. Under this circumstance, we design and implement a video based traffic flow detection system which is called MyTD in this paper. MyTD takes advantages of both shadow removal and optical(More)
and George Witman. (2003). Oda5p, a novel axonemal protein required for assembly of ; the outer dynein arm and an associated adenylate kinase. Mol. Bioi. Cell. ODACKNOWLEDGMENTS I would like to first thank my advisor, George Witman, PhD , for being an outstanding mentor, scientist and colleague. He provided a work environment that was both productive and(More)