Learn More
IgG monoclonal antibodies (mAbs) consist of two Fab fragments and one Fc fragment. The Fab fragments contain the variable regions and are responsible for drug specificity (via antigen binding); the Fc fragment contains constant regions and is responsible for effector functions (via interactions with Fcγ receptors) and extended serum half-life (via(More)
As a key component in many computer vision systems, optical flow estimation, especially with large displacements, remains an open problem. In this paper we present a simple but powerful matching method works in a coarse-to-fine scheme for optical flow estimation. Inspired by the nearest neighbor field (NNF) algorithms, our approach, called CPM(More)
—Spectral unmixing amounts to automatically finding the signatures of pure spectral components (called endmembers in the hyperspectral imaging literature) and their associated abundance fractions in each pixel of the hyperspectral image. Many algorithms have been proposed to automatically find spectral endmembers in hyperspectral data sets. Perhaps one of(More)
The import of disaccharides by many bacteria is achieved through their simultaneous translocation and phosphorylation by the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). The imported phospho-disaccharides are, in some cases, subsequently hydrolyzed by members of the unusual glycoside hydrolase family GH4. The GH4 enzymes, occasionally(More)
This study explores the use of the graphics processing units (GPUs) for performing the two-dimensional discrete wavelet transform (DWT) of images. The study of fast wavelet transforms has been driven both by the enormous volumes of data produced by modern cameras and by the need for real-time processing of these data. With the emergence of general computing(More)
Methionine oxidation commonly occurs in the Fc fragment of therapeutic monoclonal antibodies; however, its impact on antibody function has not been addressed. Using surface plasmon resonance and cell binding assays, we examined the impact of methionine oxidation on the binding of two humanized IgG1 antibodies to Fc gamma receptors (Fc gammaR) and to the(More)
PURPOSE Excipients are added to lyophilized protein drug formulations to protect the protein during processing and storage, but the mechanisms are poorly understood. Here, hydrogen/deuterium (H/D) exchange with mass spectrometry was used to assess protein conformation and excipient interactions in lyophilized solids. METHODS Calmodulin (CaM, 17 kD) was(More)
OBJECTIVE To compare video-assisted thoracoscopic surgery (VATS) lobectomy and conventional open lobectomy in patients with pulmonary tuberculosis (TB) who require surgery. METHODS Forty patients with pulmonary TB who required lobectomy were randomized to receive either VATS or open lobectomy. Patient demographic, pulmonary function, operative, and(More)
We have adapted hydrogen/deuterium (H/D) exchange with electrospray ionization mass spectrometry (ESI-MS) to study protein conformation and excipient interactions in lyophilized solids. Using calmodulin (CaM, 17 kD) as a model protein, we demonstrate that trehalose and calcium exert site-specific effects on protein conformation. The effects of calcium are(More)