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Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05
Abstract Although the lipase of Geotrichum candidum has been extensively reported, little attention has been focused on molecular genetic and biochemical characterizations of Galactomyces geotrichum… Expand
Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63
Abstract A lipase gene lipA and its chaperone gene lipB were cloned from Burkholderia cepacia strain G63. The lipA was composed of 1092 bp, encoding 363 amino acid residues, and the lipB composed of… Expand
A new extracellular thermo-solvent-stable lipase from Burkholderia ubonensis SL-4: Identification, characterization and application for biodiesel production
Abstract In the present study, a new lipase SL-4 from Burkholderia ubonensis SL-4, was purified by 80% ammonium sulphate precipitation, Q Sepharose FF anion exchange and Superdex 75 gel filtration… Expand
Effect of pretreatment by different organic solvents on esterification activity and conformation of immobilized Pseudomonas cepacia lipase
Abstract Experiments were carried out to investigate the effect of pretreatment by organic solvents with different hydrophobicities, functional groups and molecular constitutions as activation agents… Expand
Enhanced production of Thermomyces lanuginosus lipase in Pichia pastoris via genetic and fermentation strategies
- Zhonggang Fang, L. Xu, Dujie Pan, Liangcheng Jiao, Z. Liu, Yunjun Yan
- Biology, Medicine
- Journal of Industrial Microbiology…
- 30 July 2014
Abstract This study attempted to enhance the expression level of Thermomyces lanuginosus lipase (TLL) in Pichia pastoris using a series of strategies. The tll gene was first inserted into the… Expand
Esterification activity and conformation studies of Burkholderia cepacia lipase in conventional organic solvents, ionic liquids and their co-solvent mixture media.
In this work, experiments were carried out to evaluate the esterification activity and conformation of lipase from Burkholderia cepacia in the selected conventional organic solvents, ionic liquids… Expand
Enhancing catalytic performance of β-glucosidase via immobilization on metal ions chelated magnetic nanoparticles.
- T. Chen, Wenjuan Yang, Yuling Guo, Renjun Yuan, L. Xu, Yunjun Yan
- Chemistry, Medicine
- Enzyme and microbial technology
- 1 September 2014
A novel magnetic Fe3O4 nanoparticles (MNPs) coupled with agarose (AMNPs) was synthesized using co-precipitation via alkaline condition and span-80 surfactants in organic solvent. Iminodiacetate was… Expand
Optimization of Lipase-Catalyzed Transesterification of Lard for Biodiesel Production Using Response Surface Methodology
Biodiesel, an alternative diesel fuel made from renewable biological resources, has become more and more attractive recently. Combined use of two immobilized lipases with complementary position… Expand
Microcystin-LR impairs zebrafish reproduction by affecting oogenesis and endocrine system.
Previous studies have shown that microcystins (MCs) are able to exert negative effects on the reproductive system of fish. However, few data are actually available on the effects of MC-LR on the… Expand
Improving catalytic performance of Burkholderia cepacia lipase immobilized on macroporous resin NKA
Abstract The lipase from Burkholderia cepacia adsorbed on macroporous resin NKA was investigated by combined strategies of bioimprinting and interfacial activation to enhance its catalytic… Expand