Learn More
Detection of metastatic cells is clinically demanded to diagnose metastasis in the early stage and access the therapeutic response to anticancer drugs. We applied phage display technology to cultured cells with different metastasis potentials and obtained four metastasis-associated peptides. The association between peptides and metastatic cells was(More)
Circulating tumor cells (CTCs) are a group of rare cancer cells that have detached from a primary tumor and circulate in the bloodstream. Herein, light addressable potentiometric sensor (LAPS) was exploited in the label-free detection of CTCs in the prostate cancer. To this end, the mouse anti-human epithelial cell adhesion molecule (anti-EpCAM) monoclonal(More)
Early diagnosis has become the most important factor influencing the cancer's curing efficiency in clinical medicine. Here we present a new way for detection of cancer markers and cancer cells based on phage-modified Light Addressable Potentiometric Sensor (phage-LAPS). Phages were immobilized on the Si(3)N(4) chip surfaces covalently. Using the(More)
Polyvinylidene fluoride (PVDF) is popular sensing material because of its unique piezoelectric characteristics. In this work an impact sensor was prepared from a sandwiched structure PVDF film, and the related detection circuits were presented. The dependence of the PVDF sensors' response on the elasticity of the supporting materials was examined and(More)
A bio-mimetic anchoring strategy based on L-3,4-dihydroxyphenylalanine (L-DOPA) was exploited to activate the surface of light addressable potentiometric sensor (LAPS), with the structure of Si(3)N(4)/SiO(2)/Si. X-Ray photoelectron spectroscopy (XPS) measurements were carried out to ascertain its existence. The protein's immobilization on L-DOPA-initiated(More)
Multi biomarkers' assays are of great significance in clinical diagnosis. A label-free multi tumor markers' parallel detection system was proposed based on a light addressable potentiometric sensor (LAPS). Arrayed LAPS chips with basic structure of Si(3)N(4)-SiO(2)-Si were prepared on silicon wafers, and the label-free parallel detection system for this(More)
A light addressable potentiometric sensor (LAPS) is a kind of silicon based semiconductor sensor, and surface modification is a fundamental problem for its application in biological fields. Graphene oxide (GO) based biochemically activated LAPS were proposed, called GO-LAPS. The GO-LAPS were applied to monitoring single strand DNA (ssDNA) probe(More)
The sensing mechanism of binding Hg(2+) into thymine-thymine (T-T) mismatched base pairs was introduced into a light-addressable potentiometric sensor (LAPS) with anti-Hg(2+) aptamer as the sensing units. Three kinds of T-rich single-strand DNA (ssDNA) chains with different spacer lengths, from 0 to 12 -CH₂ groups, were designed to investigate surface(More)
Light addressable Potentiometric sensors (LAPS) are the biochemically sensitive semiconductor devices. Its working principles, the charge-distribution models of the electrolyte-insulator (EI) interface and its incorporation with semiconductor's photoelectric effects, are summarized. Then back illuminated LAPS with the structure of(More)
Here the phage probe as the bio-sensitive film for Light Addressable Potentiometric Sensor (LAPS) is studied. The N-terminal of the phage probes were covalently fixed on the surface of LAPS. Using the back-illuminating system the phage- LAPSs were tested to detect Human Phosphatase of Regenerating Liver-3 (hPRL-3) and the mammary adenocarcinoma cell(More)