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Virus-induced gene silencing in tomato.
TLDR
It is shown that recombinant TRV infects tomato plants and induces efficient gene silencing, and a modified TRV vector based on the GATEWAY recombination system is constructed, allowing restriction- and ligation-free cloning of tomato ESTs. Expand
Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus.
TLDR
EDS1-like gene requirement for the N function suggests that EDS1 could be a common component of bacterial, fungal and viral resistance signalling mediated by the TIR-NBS-LRR class of resistance proteins. Expand
Autophagy Regulates Programmed Cell Death during the Plant Innate Immune Response
TLDR
Plant BECLIN 1, an ortholog of the yeast and mammalian autophagy gene ATG6/VPS30/beclin 1, functions to restrict HR PCD to infection sites and plays an essential role in plant innate immunity and negatively regulates PCD. Expand
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms. Expand
The Jasmonate-ZIM Domain Proteins Interact with the R2R3-MYB Transcription Factors MYB21 and MYB24 to Affect Jasmonate-Regulated Stamen Development in Arabidopsis[C][W]
TLDR
This work shows that the R2R3-MYB transcription factors MYB21 and MYB24 function as direct targets of JAZ proteins to mediate jasmonate-regulated stamen development and regulate male fertility specifically. Expand
Virus-induced gene silencing.
TLDR
The use of tobacco rattle virus-based VIGS technique is described to study gene function in Nicotiana benthamiana and tomato. Expand
Role of SCF Ubiquitin-Ligase and the COP9 Signalosome in the N Gene–Mediated Resistance Response to Tobacco mosaic virus Article, publication date, and citation information can be found at
TLDR
It is shown that the NbRar1 protein interacts with NbSGT1, a highly conserved component of the SCF (Skp1/Cullin/F-box protein)-type E3 ubiquitin ligase complex involved in protein degradation. Expand
Efficient Virus-Induced Gene Silencing in Arabidopsis1
TLDR
It is shown that VIGS can be used to silence genes involved in general metabolism and defense and it is also effective at knocking down expression of highly expressed transgenes. Expand
Molecular Chaperone Hsp90 Associates with Resistance Protein N and Its Signaling Proteins SGT1 and Rar1 to Modulate an Innate Immune Response in Plants*
TLDR
The results reveal a new role for SGT1- and Rar1-associated chaperone machinery in R gene-mediated defense signaling and show that Hsp90 associates with the resistance protein N that confers resistance to tobacco mosaic virus. Expand
Role of a novel type of double infection in the geminivirus‐induced epidemic of severe cassava mosaic in Uganda
TLDR
The evidence indicates that UgV is probably of relatively recent origin, that such variants do not appear often, and that the current epidemic has resulted from the rapid spread of UgV to infect plants and to invade regions in which ACMV already occurred. Expand
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