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We designed a new inverse PCR protocol combined with switching mechanism at 5' end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5'- and 3'-ends of cDNA in a single reaction, and might(More)
This paper deals with characteristics of accommodation evoked by perceived depth sensation and the dynamic relationship between accommodation and vergence, applying newly developed optical measurement apparatuses. A total of five subjects looked at three different two-dimensional stimuli and two different three-dimensional stimuli; namely a real image and a(More)
In interactive applications such as games, particle-based liquids are often used for simulation. Such liquids are rendered with only a single refraction at the front-facing surface, which damages photorealism because it cannot express the thickness of liquid. In this paper, we propose an approach to render particle-based liquid with twice refraction at(More)
In most surgical training systems, task completion time and error ratio are common metrics of surgical skill. To avoid applying unnecessary and injurious force to the tissue, surgeons must know for themselves how much force they are exerting as they handle surgical tools. Our goal is to develop an endoscopic sinus surgery (ESS) training system that(More)