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We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 microg of total RNA) without(More)
alpha-Glucosidase I of Bacillus thermoamyloliquefaciens KP1071 (FERM P8477, facultative thermophile) was purified to homogeneity. The relative molecular mass was estimated to be 62,000 Da. From its catalytic properties, the enzyme has been assigned to an exo-alpha-1,4-glucosidase. The enzyme shares its antigenic groups in part with Bacillus(More)
The respiratory activity of collagen-embedded living cells was imaged by scanning electrochemical microscopy (SECM) with the objective to study anticancer drug sensitivity. Two kinds of cancer cells, the human erythroleukemia cell line (K562) and its adriamycin-resistant subline (K562/ADM), were immobilized at the array of microholes micromachined on a(More)
The synthesis and structural analysis of the hetero-bimetallic M-Al complexes MMe[(O-2,4-(t)Bu2C6H2-6-CH2)2(μ2-O-2,4-(t)Bu2C6H2-6-CH2)N][Me2Al(μ2-O(i)Pr)] [M = Zr, Hf], prepared from M(O(i)Pr)[{(O-2,4-(t)Bu2C6H2)-6-CH2}3N] by treating with 1.0 equiv. of AlMe3, have been explored: these complexes exhibited catalytic activities for ethylene polymerisation in(More)
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