Yuki Shiba

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Many studies have suggested that 5-hydroxytryptamine (5HT, serotonin) plays an important role in the control of gastrointestinal motility. However, most of these studies have been carried out on guinea-pig ileum in vitro. Therefore, we investigated the mechanisms of action of 5HT on gastrointestinal motility in conscious guinea-pigs. In order to investigate(More)
In general, anchorage-dependent animal cells cultivated on a solid culture substrate, such as polystyrene, are collected by trypsin treatment. This treatment may have detrimental effects such as the proteolysis of the cell membrane proteins. To avoid these effects, cell cultivation using a liquid/liquid interface system has been investigated. In this(More)
We compared the inhibitory effects of histamine and pentagastrin (PG) on motilin-induced upper gastrointestinal phase III activity in conscious dogs that had surgically prepared Heidenhain pouchs (HP). Contractile activity was measured by means of chronically implanted force transducers, and changes in pH of the perfusate through the HP were monitored(More)
BACKGROUND & AIMS EM523, a motilin agonist, is intended to be used as a gastroprokinetic during the postprandial period, but the mechanism(s) by which EM523 stimulates postprandial contractions in the stomach has not been studied before. The aim of this study was to examine the mechanism of contraction-stimulating activity by EM523 in fed dogs. METHODS(More)
Mixed-function oxidase activities of hepatic microsomal preparations from rats were examined after intraperitoneal administration of sizofilan (SPG), an immunomodulator. Repeated doses of SPG (3 mg/kg/12 hr, 4 times) depressed the hepatic cytochrome P-450 content and the activities of aminopyrine N-demethylase and aniline hydroxylase.
The central projection model commonly used to model cameras as well as projectors, results in similar advantages and disadvantages in both types of system. Considering the case of active stereo systems using a projector and camera setup, a central projection model creates several problems, among them, narrow depth range and necessity of wide baseline are(More)
BACKGROUND & AIMS EM523, a nonpeptide motilin agonist, is being developed for clinical use to improve delayed gastric emptying. The aim of this study was to examine the effect of EM523 on endogenous release of gut and pancreatic hormones in conscious dogs. METHODS Motility of the gastrointestinal tract was monitored using force transducers. Blood(More)
Sex related differences in drugs metabolizing enzymes was studied using Wistar-rats bearing carrageenan-induced edema. One day after treatment with carrageenan, aminopyrine N-demethylase activity was inhibited in males but not in females. Carrageenan treatment did not alter the activity of aniline hydroxylase in either sex of the rats. These data show that(More)