Yuji Shibano

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We purified a novel α-glucosidase to homogeneity from an Escherichia coli recombinant transformed with the α-glucosidase gene from thermophilic Bacillus sp. SAM1606. The enzyme existed as mono- and multimeric forms of a promoter protein with a relative molecular weight of 64,000 and isoelectric point of 4.6. We isolated a monomeric form of the enzyme and(More)
BACKGROUND We examined the influences of a moderate intake level of three types of alcoholic beverages--beer, whisky, and Shochu (Japanese distilled liquor)--on purine and carbohydrate metabolism and excretion in healthy male volunteers, concerning (1) the extent of contribution of purine bodies contained in beer to uric acid metabolism and (2) a comparison(More)
The expression and secretion of Rhizopus oryzae glucoamylase were studied in the yeast Saccharomyces cerevisiae. Rhizopus oryzae glucoamylase was highly expressed and efficiently secreted into a medium to a high level (above 300 mg/l) under control of a yeast promoter and the original signal sequence. Excess expression of the secretable glucoamylase with(More)
This paper provides an experimental discussion on zero-voltage-ride-through (ZVRT) capability of a phase-shifted pulsewidth-modulation distribution static synchronous compensator (D-STATCOM) using the modular multilevel cascade converter based on single-star bridge cells (SSBC). The cluster-balancing control producing a significant effect on the ZVRT(More)
This paper presents and discusses a phase-shifted-PWM distribution static synchronous compensator (D-STATCOM) using the modular multilevel cascade converter based on single-star bridge cells (MMCC-SSBC). A three-phase 140-V 10-kVA SSBC-based STATCOM with a cascade count N=6 is designed, constructed, and tested for verifying the performance and functionality(More)
This paper has an experimental discussion on low-voltage-ride-through (LVRT) capability of a phase-shifted-PWM STATCOM using the modular multilevel cascade converter based on single-star bridge-cells (MMCC-SSBC). The cluster-balancing control, that produces a significant effect on the LVRT capability, is modeled and analyzed with focus on either low-pass(More)
We purified an extracellular thermostable β-galactosidase of Saccharopolyspora rectivirgula strain V2-2, a thermophilic actinomycete, to homogeneity and characterized it to be a monomeric enzyme with a relative molecular mass of 145 000 and s°20,w of 7.1 s. In addition to the hydrolytic activity of 1-O-substituted β-d-galactopyranosides such as lactose [a(More)
The monomeric multimetal-binding beta-galactosidase of Saccharopolyspora rectivirgula (srbg), a glycosyl hydrolase family-2 enzyme, has a unique sequence consisting of 192 amino acid residues with no similarity to known proteins. This 192-residue sequence (termed the "iota [iota] sequence") appears to be inserted into a sequence homologous to the(More)
This paper presents and discusses a phase-shifted-PWM STATCOM using the modular multilevel cascade converter based on single-star bridge-cells (MMCC-SSBC). A three-phase 140-V, 10-kVA downscaled experimental system with a cascade count N =6 is designed, constructed and tested for verifying the performance and functionality of the STATCOM on both steady and(More)
toxin produced by fungi in the genera Fusarium and Gibber-ella.lf2) It alters the permeability of plant cytoplasmic membranesto water and causes solute leakage from tomato leaves.3A) It also chelate copper and other ions that could be involved in maintaining membrane integrity5'^ and affects membrane potentials and ATP levels of tomato root hair cells.7)(More)
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