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Dissertação apresentada para obtenção do Grau de Doutor em Engenharia do Ambiente, ABSTRACT Genetic algorithms (GAs) have been used to solve difficult optimization problems in a number of fields. One of the advantages of these algorithms is that they operate well even in domains where little is known, thus giving the GA the flavor of a general purpose(More)
PURPOSE To examine the feasibility of cultivated corneal endothelial cell transplantation in a primate model. METHODS Monkey corneal endothelial cells (MCECs) obtained from three cynomolgus monkeys were cultivated, with subcultures grown on collagen type I carriers for 4 weeks. The corneal endothelium of the right eye of six monkeys was mechanically(More)
PURPOSE The transplantation of cultivated corneal endothelial cells (CECs) has gained attention recently for the treatment of patients with corneal endothelial dysfunction. However, an efficient culturing technique for human (H)CECs has yet to be properly established. The present study was conducted to investigate the applicability of the Rho kinase (ROCK)(More)
PURPOSE To evaluate the effect of Rho kinase (ROCK)-inhibitor eye drops on a corneal endothelial dysfunction primate model and human clinical case series of corneal endothelial dysfunction. METHODS As a corneal-endothelial partially injured model, the corneal endothelium of seven cynomolgus monkeys was damaged by transcorneal freezing; 10 mm of rock(More)
In recent years, an increased effort to design, build, and operate small satellites has taken place in universities and laboratories all over the world. These micro-satellites (or nano-satellites) provide numerous flight opportunities for science experiments at a fraction of the cost of larger traditional missions. In addition, there has been an increasing(More)
PURPOSE Our study examined the effect of a selective Rho kinase inhibitor, Y-27632, on corneal wound healing and potential stromal scarring after superficial keratectomy. METHODS Rabbit keratocytes were induced into myofibroblasts by transforming growth factor β1 (TGFβ1) either with or without Y-27632. Then α-smooth muscle actin (α-SMA) was examined by(More)
PURPOSE To investigate the possibility of using polylactic acid (PLA) as a surgical adjuvant for visualizing the vitreous body during vitrectomy. METHODS After a core vitrectomy, 1 mL of PLA suspension was injected into the rabbit vitreous in two groups: group A, 2.5% PLA (n = 5), and group B, 1% PLA (n = 9). Vehicle injection instead of PLA was used as a(More)
The corneal endothelium maintains corneal transparency; consequently, its dysfunction causes severe vision loss. Tissue engineering-based therapy, as an alternative to conventional donor corneal transplantation, is anticipated to provide a less invasive and more effective therapeutic modality. We conducted a preclinical study for cell-based therapy in a(More)