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Criteria for Annotation of Plant MicroRNAs
TLDR
The specific criteria required for the annotation of plant miRNAs are updated, including experimental and computational data, as well as refinements to standard nomenclature. Expand
Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions.
TLDR
It was demonstrated that Arabidopsis Dicer homologue Dicer-like 1 (DCL1) catalyzes at least the first and second cleavage steps and that double-stranded RNA-binding domains of DCL1 are involved in positioning of the cleavage sites, direct evidence that DCL 1 is involved in processing of pri- and pre-miRNA. Expand
The mechanism selecting the guide strand from small RNA duplexes is different among argonaute proteins.
TLDR
The results clearly indicate that the mechanism selecting the guide strand is different among AGO proteins and that multiple AGO genes are involved in anti-virus defense in plants. Expand
Tobacco mosaic virus infection spreads cell to cell as intact replication complexes.
TLDR
A model whereby cell-cell spread of tobamovirus infection is accomplished by subviral replication complexes that initiate TMV replication immediately after entry to adjacent cells is proposed. Expand
Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
TLDR
Improved pGWBs should be a powerful tool for transgenic research in plants because of their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Expand
The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis.
TLDR
Results indicate that HYL1 has a function in assisting efficient and precise cleavage of pri-miRNA through interaction with DCL1. Expand
Activation of peroxisome proliferator-activated receptor delta induces fatty acid beta-oxidation in skeletal muscle and attenuates metabolic syndrome.
TLDR
Analysis of rat L6 myotubes treated with the PPARdelta subtype-selective agonist, GW501516, by the Affymetrix oligonucleotide microarrays revealed that PPardelta controls fatty acid oxidation by regulating genes involved in fatty acid transport, beta-oxidation, and mitochondrial respiration. Expand
Activation of peroxisome proliferator-activated receptor δ induces fatty acid β-oxidation in skeletal muscle and attenuates metabolic syndrome
TLDR
Analysis of rat L6 myotubes treated with the PPARδ subtype-selective agonist, GW501516, by the Affymetrix oligonucleotide microarrays revealed that PPAR Δ is pivotal to control the program for fatty acid oxidation in the skeletal muscle, thereby ameliorating obesity and insulin resistance through its activation in obese animals. Expand
UPF3 suppresses aberrant spliced mRNA in Arabidopsis.
TLDR
The AtUPF3 gene is required by the plant NMD system to obviate aberrantly spliced mRNA, indicating that the atupf3 mutant was less able to degrade mRNAs with premature termination codons than wild-type plants. Expand
SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6‐bodies
TLDR
RDR6 and SGS3 colocalize by fluorescence microscopy for Q9LDX1/Q9SG02 and RDR6/SGS3 co-localization is confirmed using uniprotkb-based approaches. Expand
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