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Ribosome rescue by Escherichia coli ArfA (YhdL) in the absence of trans‐translation system
Although SsrA(tmRNA)‐mediated trans‐translation is thought to maintain the translation capacity of bacterial cells by rescuing ribosomes stalled on messenger RNA lacking an in‐frame stop codon,Expand
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Escherichia coli YaeJ protein mediates a novel ribosome‐rescue pathway distinct from SsrA‐ and ArfA‐mediated pathways
Accumulation of stalled ribosomes at the 3′ end of mRNA without a stop codon (non‐stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescueExpand
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ArfA recruits release factor 2 to rescue stalled ribosomes by peptidyl‐tRNA hydrolysis in Escherichia coli
The ribosomes stalled at the end of non‐stop mRNAs must be rescued for productive cycles of cellular protein synthesis. Escherichia coli possesses at least three independent mechanisms that resolveExpand
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trans-translation-mediated tight regulation of the expression of the alternative ribosome-rescue factor ArfA in Escherichia coli.
Ribosomes translating mRNA without an in-frame stop codon (non-stop mRNA) stall at its 3' end. In eubacteria, such ribosomes are rescued by SsrA-mediated trans-translation. Recently, we have shownExpand
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ArfA recognizes the lack of mRNA in the mRNA channel after RF2 binding for ribosome rescue
Although trans-translation mediated by tmRNA-SmpB has long been known as the sole system to relieve bacterial stalled ribosomes, ArfA has recently been identified as an alternative factor forExpand
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Nascentome Analysis Uncovers Futile Protein Synthesis in Escherichia coli
Although co-translational biological processes attract much attention, no general and easy method has been available to detect cellular nascent polypeptide chains, which we propose to callExpand
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The fail-safe system to rescue the stalled ribosomes in Escherichia coli
Translation terminates at stop codon. Without stop codon, ribosome cannot terminate translation properly and reaches and stalls at the 3′-end of the mRNA lacking stop codon. Bacterial tmRNA-mediatedExpand
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Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing
Significance The synthesis of a protein takes tens of seconds to a few minutes, in which amino acids are polymerized linearly. Nonuniform progression of this elongation process is thought to beExpand
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Intrinsic Ribosome Destabilization Underlies Translation and Provides an Organism with a Strategy of Environmental Sensing.
Nascent polypeptides can modulate the polypeptide elongation speed on the ribosome. Here, we show that nascent chains can even destabilize the translating Escherichia coli ribosome from within. ThisExpand
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Escherichia coli small heat shock protein IbpA is an aggregation-sensor that self-regulates its own expression at post-transcriptional levels.
Aggregation is an inherent characteristic of proteins. Risk management strategies to reduce aggregation are critical for cells to survive upon stresses that induce aggregation. Cells cope withExpand