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Galacto-oligosaccharides (GOS), an important class of functional food, are commonly produced from lactose using β-galactosidase. In the present study, β-galactosidase (LacS) from Sulfolobus solfataricus P2 was cloned and site-directed mutagenesis was performed to obtain two mutants, F359Q and F441Y. All of the wild-type enzyme and mutants were expressed in(More)
OBJECTIVE Alpha-glucosidase from Aspergillus niger SG136 was expressed in Pichia pastoris. METHODS cDNA of mature alpha-glucosidase (aglu) was amplified from the total DNA of A. niger SG136 by PCR and overlap-PCR with primers designed based on the sequence of A. niger CBS 513.88 in NCBI database. The gene was cloned into pMD18-T simple vector. The(More)
In this paper, the statistical analysis of cluster is presented based on the outdoor wideband multiple-input multiple-output (MIMO) channel measurements to facilitate IMT-Advanced system design. Clusters are found in multi-dimensional space, i.e., on the azimuth of arrival-azimuth of departure-elevation of departure-delay domain. We identify clusters with(More)
RATIONALE Cold-inducible RNA-binding protein (CIRP) is constitutively expressed at low levels across various tissues. It is rapidly upregulated by multiple stresses, underlying a general role for CIRP in organic adaptations to pathophysiological conditions. However, the role of CIRP in the heart remains unclear. OBJECTIVE To examine the biofunctions of(More)
Cardiac arrhythmias are among the most common causes of death in the world. Foundational studies established the critical role of ion channel disorders in arrhythmias, yet defects in ion channels themselves, such as mutations, may not account for all arrhythmias. Despite the progress made in recent decades, the antiarrhythmic drugs currently available have(More)
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