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OBJECTIVE Alpha-glucosidase from Aspergillus niger SG136 was expressed in Pichia pastoris. METHODS cDNA of mature alpha-glucosidase (aglu) was amplified from the total DNA of A. niger SG136 by PCR and overlap-PCR with primers designed based on the sequence of A. niger CBS 513.88 in NCBI database. The gene was cloned into pMD18-T simple vector. The(More)
Galacto-oligosaccharides (GOS), an important class of functional food, are commonly produced from lactose using β-galactosidase. In the present study, β-galactosidase (LacS) from Sulfolobus solfataricus P2 was cloned and site-directed mutagenesis was performed to obtain two mutants, F359Q and F441Y. All of the wild-type enzyme and mutants were expressed in(More)
RATIONALE Cold-inducible RNA-binding protein (CIRP) is constitutively expressed at low levels across various tissues. It is rapidly upregulated by multiple stresses, underlying a general role for CIRP in organic adaptations to pathophysiological conditions. However, the role of CIRP in the heart remains unclear. OBJECTIVE To examine the biofunctions of(More)
1 Cold-inducible RNA-binding protein (CIRP) is an 18-kDa protein consisting of an amino-terminal RNA-binding domain and a carboxyl-terminal glycine-rich domain. The expression of Cirp was first identified in murine germ cells, a cell type naturally exposed to temperatures lower than body temperature. 2 Subsequent studies reveal that Cirp is expressed in a(More)
Cardiac arrhythmias are among the most common causes of death in the world. Foundational studies established the critical role of ion channel disorders in arrhythmias, yet defects in ion channels themselves, such as mutations, may not account for all arrhythmias. Despite the progress made in recent decades, the antiarrhythmic drugs currently available have(More)
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