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The GIT-associated kinase PAK targets to the centrosome and regulates Aurora-A.
- Zhuo-shen Zhao, J. P. Lim, Yuen-Wai Ng, L. Lim, E. Manser
- Biology, Medicine
- Molecular cell
- 28 October 2005
Previously, we showed PAK-PIX-GIT targets and regulates focal adhesions; here, we uncover a different function for the complex at the centrosome. Active PAK1 is particularly evident in mitosis and… Expand
GIT1 Activates p21-Activated Kinase through a Mechanism Independent of p21 Binding
- T. Loo, Yuen-Wai Ng, L. Lim, E. Manser
- Biology, Medicine
- Molecular and Cellular Biology
- 1 May 2004
ABSTRACT p21-activated kinases (PAKs) associate with a guanine nucleotide exchange factor, Pak-interacting exchange factor (PIX), which in turn binds the paxillin-associated adaptor GIT1 that targets… Expand
Group I and II mammalian PAKs have different modes of activation by Cdc42
- Yohendran Baskaran, Yuen-Wai Ng, Widyawilis Selamat, Felicia Tay Pei Ling, E. Manser
- Biology, Medicine
- EMBO reports
- 1 July 2012
p21‐activated kinases (PAKs) are Cdc42 effectors found in metazoans, fungi and protozoa. They are subdivided into PAK1‐like (group I) or PAK4‐like (group II) kinases. Human PAK4 is widely expressed… Expand
Paxillin nuclear-cytoplasmic localization is regulated by phosphorylation of the LD4 motif: evidence that nuclear paxillin promotes cell proliferation.
- Jing-ming Dong, Lei-Shong Lau, Yuen-Wai Ng, L. Lim, E. Manser
- Biology, Medicine
- The Biochemical journal
- 15 February 2009
Paxillin, a major focal-adhesion complex component belongs to the subfamily of LIM domain proteins and participates in cell adhesion-mediated signal transduction. It is implicated in cell-motility… Expand
Why an A-loop phospho-mimetic fails to activate PAK1: understanding an inaccessible kinase state by molecular dynamics simulations.
- Yuen-Wai Ng, Devanathan Raghunathan, +5 authors E. Manser
- Biology, Medicine
- Structure
- 14 July 2010
Crystal structures of inactive PAK1(K299R) and the activation (A)-loop phospho-mimetic PAK1(T423E) have suggested that the kinase domain is in an active state regardless of activation loop status.… Expand
A Robust Protocol to Map Binding Sites of the 14-3-3 Interactome: Cdc25C Requires Phosphorylation of Both S216 and S263 to bind 14-3-3*
- P. M. Chan, Yuen-Wai Ng, E. Manser
- Biology, Medicine
- Molecular & Cellular Proteomics
- 12 December 2010
Modern proteomic techniques have identified hundreds of proteins that bind 14-3-3s, the most widespread eukaryotic phosphoserine/threonine sensors, but accurate prediction of the target phospho-sites… Expand
Group-I PAKs-mediated phosphorylation of HACE1 at serine 385 regulates its oligomerization state and Rac1 ubiquitination
- Maria I. Acosta, S. Urbach, +7 authors E. Lemichez
- Chemistry, Medicine
- Scientific Reports
- 23 January 2018
The regulation of Rac1 by HACE1-mediated ubiquitination and proteasomal degradation is emerging as an essential element in the maintenance of cell homeostasis. However, how the E3 ubiquitin ligase… Expand
IImammalian PAKs have differentmodes of activation by Cdc 42
p21-activated kinases (PAKs) are Cdc42 effectors found in metazoans, fungi and protozoa. They are subdivided into PAK1like (group I) or PAK4-like (group II) kinases. Human PAK4 is widely expressed… Expand