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The role of Asp-212 in the proton pumping mechanism of bacteriorhodopsin (bR) has been studied by a combination of site-directed mutagenesis and Fourier transform infrared difference spectroscopy.(More)
Fourier transform infrared difference spectroscopy has been used to study the photocycle of the mutant Tyr-185-->Phe expressed in native Halobacterium halobium and isolated as intact purple membrane(More)
Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy,(More)
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