Yuanshe Sun

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A strategy of dimethylamino alkyldiimide conjugated with an intercalator of naphthalimide for hydrolysis of DNA was suggested and evaluated. 4 can hydrolyze 4 kb plasmid DNA into 2 kb fragments with GC and GG selectivity, which represents a novel example of sequence- or site-selective metal-free DNA artificial nuclease. Results also show it could hydrolyze(More)
A new kind of silica-bonded reserved stationary phase with s-triazine and amide polar groups embedded for HPLC was obtained by step-wise reactions of silica gel with s-triazine, gamma-aminopropyltriethoxysilane, ethanediamine and dodecanoyl chloride. The new stationary phase was characterized by elemental analysis. After that, a column packed with the new(More)
Thio-heterocylic naphthalimides (R1-R5) were designed, synthesized and evaluated as nonmetallic and long-wavelength photocleavers. Some of them showed highly efficient abilities in the degradation of plasmid and genomic DNA under the mild conditions without obvious impairment on the proteins' bioactivities, when compared with frequently applied nucleic(More)
A method of quantitative analysis of sulfur amino acids in feedstuffs by high performance liquid chromatography coupled with pre-column derivatization was developed. Before the feedstuffs were hydrolyzed under acidic condition, the cystine and methionine in the feedstuffs were oxidized to cysteic acid and methionine sulfone respectively by performic acid,(More)
A sensitive high performance liquid chromatography (HPLC)-laser induced fluorescence detection (LIFD) method was developed for the determination of amines. The derivatization and separation conditions were investigated. Under the optimized conditions, spermidine, putrescine and histamine were analyzed. The limits of detection (LODs) of the three biogenic(More)
A simple and effective sample enrichment method of frozen zone melting liquid-liquid extraction was optimized and validated for the analysis of trace N-nitrosodimethylamine (NDMA) in beer samples. The method was based on high pressure liquid-liquid extraction with a low temperature frozen step. The 90 mL beer was placed in a container with 10 mL(More)
Laser-induced fluorescence detector (LIFD) is one of the most sensitive detectors in analytical chemical files. Confocal optical configuration is widely used in LIFDs. Two effective approaches used to achieve the best signal to noise ratio (S/N) are increasing the confocal precision and minimizing the background noise. A novel three-dimensional adjustable(More)
With the development of science and technology, more and more complex samples like peptides and proteins need to be separated. It is difficult to separate them by single mode chromatography. Due to the unique separation character of mixed-mode chromatography, the same separation performance can be obtained in mixed-mode chromatography in one separation as(More)
A method of high performance liquid chromatography (HPLC) was developed for the separation and determination of six acidic components (cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone) in hops extracts. The effects of several important factors, such as the addition of acid, the organic solvent of elution solution and the column(More)
We have designed and synthesized novel intercalating compounds that efficiently hydrolyze supercoiled plasmid DNA lacking depurine/depyrimidine sites. These intercalators consist of conjugates of diamine and thia-heterocyclic naphthalimide. Surprisingly, the initial hydrolyzed products comprised fragments with half the length of the original plasmid. We(More)
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