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Cloning and expression of a new rat procarboxypeptidase B gene in Escherichia coli and purification of recombination carboxypeptidase B.
A new coding sequence of the procarboxypeptidase B gene was obtained from SD rat fresh pancreas by RT-PCR and highly expressed in Escherichia coli in inclusion bodies and it was shown that the obtained recombinant carboxypepticase B could substitute carboxypesptidases B isolated from Pancreas. Expand
The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B.
A new pro-carboxypeptidase (pCPB) gene was cloned by RT-PCR from SD rat pancreas and its overexpression in Escherichia coli resulted in the formation of inclusion bodies (IBs). The IBs of pCPB wereExpand
Identification of protein c activator from nine species of Chinese snake venoms
AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastinExpand
Study on Purification Condition of Recombinant Human Manganese Superoxide Dismutase
Objective To study the purification method of recombinant human manganese superoxide dismutase(rhMn-SOD).Methods The cells were fragmented by ultrasonic.The protein was precipitated by the thermalExpand
Isolation and Purification of Superoxide Dismutase from Radix Lithospermi Seed
Superoxide dismutase (SOD) (EC was purified to homogeneity, from Radix Lithospermi seeds (RLS). Lipid and other pigments were removed by super critical fluid extraction (SCF). StepwiseExpand
High Cell-Density Culture of E.coli TB1/pMAL-hOCIFm
Batch and fed-batch culture process of E.coli TB1/pMAL-hOCIFm were carried out to produce recombinant human osteoclastogenesis inhibitory factor mature peptide (rhOCIFm) in 3.7 L BioengineeringExpand
Cloning and expression of new apoptin fusion protein and its bioactivity.
Objective To clone and express apoptin-EC-SOD3 fusion protein and determine its bioactivity in vitro.Methods Apoptin gene was amplified by PCR and cloned into prokaryotic expression vectorExpand
Screening of bioactive products in twelve kinds of marine animals from Zhoushan archipelago in Zhejiang province
The extracts from Solen grandis Dunker and Bullacta exarata with ethanol and Haliplanella luciae with water showed cytotoxic activity, the IC 50 of which against SMMU-7721 was 89,71,63.2μg·mL -1, respectively. Expand
Pro-sequence Engineering
Pro-sequence engineering refers to an identical polypeptide can fold into an altered conformation with different secondary structure, stability and specificities through a mutated pro-sequence, which is not only an important tool for studying the mechanism of protein folding, but also a promising technolique for creating unique proteases with various beneficial properties. Expand