• Citations Per Year
Learn More
The efficient expression of small to midsize polypeptides and small marginally stable proteins can be difficult. A new protein fusion system is developed to allow the expression of peptides and small proteins. The polypeptide of interest is linked via a Factor Xa cleavage sequence to the C-terminus of the N-terminal domain of the ribosomal protein L9(More)
The helical subdomain of the villin headpiece is the smallest naturally occurring cooperatively folded protein. Its small size, simple three-helix topology, and very rapid folding have made it an extremely popular model system for computational and theoretical studies of protein folding. The domain has a well-packed hydrophobic core comprised in part of an(More)
The 36-residue helical subdomain of the villin headpiece, HP36, is one of the smallest cooperatively folded proteins, folding on the microsecond time scale. The domain is an extraordinarily popular model system for both experimental and computational studies of protein folding. The structure of HP36 has been determined using X-ray crystallography and NMR(More)
Aiming at the data sparse and cold start problems in collaborative filtering recommendation algorithm, an optimized solution based on user characteristics and user ratings is proposed in this paper. Based on users' basic attributes and users' history score record, the similarity of users and the similarity of items are calculated, and the nearest neighbor(More)
The alpha-lactalbumins and c-type lysozymes have virtually identical structure but exhibit very different folding behavior. All alpha-lactalbumins form a well populated molten globule state, while most of the lysozymes do not. alpha-Lactalbumin consists of two subdomains, and the alpha-subdomain is considerably more structured in the molten globule state(More)
Lots of toolboxes of accelerating MatLab using GPU are available now[1], but, users are confused by which toolbox is best suitable for a particular task. Three toolboxes—Jacket, GPUmat, and Parallel Computing Toolbox of MatLab are selected. For each toolbox, its advantages and pitfalls are reviewed, with an aim to allow the reader to identify which(More)
The rational modification of protein stability is an important goal of protein design. Protein surface electrostatic interactions are not evolutionarily optimized for stability and are an attractive target for the rational redesign of proteins. We show that surface charge mutants can exert stabilizing effects in distinct and unanticipated ways, including(More)
A hyperstable variant of the small independently folded helical subdomain (HP36) derived from the F-actin binding villin headpiece was designed by targeting surface electrostatic interactions and helical propensity. A double mutant N68A, K70M was significantly more stable than wild type. The Tm of wild type in aqueous buffer is 73.0 degrees C, whereas the(More)
  • 1