Yu-qiu Zhu

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OBJECTIVE To investigate HSP70 gene expression from Dendrobium officinale under low temperature stress, which will provide the molecular biological foundation for breeding the low temperature resistant strain. METHOD HSP70 gene full length cDNA was cloned by rapid amplification of cDNA ends (RACE) on the basis of HSP70 gene fragment sequences, and the(More)
OBJECTIVE To compare the different extraction methods of Sarcandra glabra (Thunb.) Nakai. METHODS To compare the yield of extraction and determination content of extraction by 6 kinds of different extraction methods as cold-soaked extraction, percolation extraction, reflux extraction, continuous reflux extraction, ultrasonic extraction and microwave(More)
The study is aimed to make the most of available space of Dendrobium officinale cultivation facility, reveal the yield and functional components variation of stereoscopic cultivated D. officinale, and improve quality, yield and efficiency. The agronomic traits and yield variation of stereoscopic cultivated D. officinale were studied by operating field(More)
Using universal primer Tyl-copia retrotransposon RT, the conserved reverse transcriptase domain of about 260 bp was amplified by RT-PCR from the Dendrobium officinale which induced by 100 micromol x L(-1) abscisic acid (ABA), indicating these retrotransposons activated by 100 micromol x L(-1) ABA. The amplicons were recovered and cloned,then sequenced and(More)
OBJECTIVE To select superior mating combinations from different F1 generations of Dendrobium officinale. METHOD Twenty-six superior parents from four provenances were selected to make up 26 mating combinations. The agronomic traits of different F1 generation were measured, counted, and analyzed by DPS software. RESULT There were significant differences(More)
OBJECTIVE To reveal the relationship between the storage time of the bark of Magnolia officinalis and the content of phenols in it, and lay a theoretical foundation for the harvest, processing, management and storage. METHOD The contents of magnolol and honokoiol in 15 bark samples, collected from the main producing areas in China, were determined in the(More)
A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and(More)
To standardize the harvest ways of Dendrobium officinale and improve the quality and yield of D. officinale, a field experiment was carried out to study the effect of two kinds of harvest ways, which were keeping some of the axial shoot and harvesting all of the shoot by the end of the year. Then, the agronomic traits and yield were measured and the(More)
The explants were obtained from stem segments of hybrid combination of Dendrobium officinale germplasms (Zhejiang Yandang Mountain x Yunnan Guangnan). The screened culture mediums for buds induction and multiplication, which were 1/2MS + IBA 1.0 mg x L(-1) +6-BA 1.0 mg x L(-1) and 1/2MS + IBA 1.5 mg x L(-1) +6-BA 0.5 mg x L(-1) respectively, were applied to(More)
To solve the issues of costly planting of facility cultivation method and inferior efficacy than wild herbs of Dendrobium officinale, the cliff epiphytic cultivation method was studied. To research the growth, agronomic traits, yield, polysaccharide and alcohol-soluble extract contents were measured on the D. officinale from different water regulation and(More)